QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

Vol. 17, Issue 6, 2799-2810, June 2006

This Article Full Text Full Text (PDF) Supplemental Material All Versions of this Article: E05-05-0450v1 E05-05-0450v2 17/6/2799    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wloga, D. Articles by Gaertig, J. Search for Related Content PubMed PubMed Citation Articles by Wloga, D. Articles by Gaertig, J.

Members of the NIMA-related Kinase Family Promote Disassembly of Cilia by Multiple Mechanisms

Dorota Wloga^*, Amy Camba^*, Krzysztof Rogowski^*,, Gerard Manning, Maria Jerka-Dziadosz, and Jacek Gaertig^*

*Department of Cellular Biology, University of Georgia, Athens, GA 30602-2607; Razavi-Newman Center for Bioinformatics, Salk Institute for Biological Studies, La Jolla, CA 92037; and Department of Cell Biology, M. Nencki Institute of Experimental Biology, Polish Academy of Science, 02-093 Warsaw, Poland

Submitted May 23, 2005; Revised March 30, 2006; Accepted April 3, 2006
Monitoring Editor: Kerry Bloom

The genome of Tetrahymena thermophila contains 39 loci encoding NIMA-related kinases (NRKs), an extraordinarily large number for a unicellular organism. Evolutionary analyses grouped these sequences into several subfamilies, some of which have orthologues in animals, whereas others are protist specific. When overproduced, NRKs of three subfamilies caused rapid shortening of cilia. Ultrastructural studies revealed that each NRK triggered ciliary resorption by a distinct mechanism that involved preferential depolymerization of a subset of axonemal microtubules, at either the distal or proximal end. Overexpression of a kinase-inactive variant caused lengthening of cilia, indicating that constitutive NRK-mediated resorption regulates the length of cilia. Each NRK preferentially resorbed a distinct subset of cilia, depending on the location along the anteroposterior axis. We also show that normal Tetrahymena cells maintain unequal length cilia. We propose that ciliates used a large number of NRK paralogues to differentially regulate the length of specific subsets of cilia in the same cell.

The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).

Present address: Centre de Recherches de Biochimie Macromoléculaire, CNRS, 34293 Montpellier, France.

Address correspondence to: Maria Jerka-Dziadosz ( dziadosz{at}nencki.gov.pl) or Jacek Gaertig (jgaertig{at}cb.uga.edu)

This article has been cited by other articles:

				D. Wloga, D. Dave, J. Meagley, K. Rogowski, M. Jerka-Dziadosz, and J. Gaertig Hyperglutamylation of Tubulin Can either Stabilize or Destabilize Microtubules in the Same Cell Eukaryot. Cell, January 1, 2010; 9(1): 184 - 193. [Abstract] [Full Text] [PDF]

				T. Piao, M. Luo, L. Wang, Y. Guo, D. Li, P. Li, W. J. Snell, and J. Pan A microtubule depolymerizing kinesin functions during both flagellar disassembly and flagellar assembly in Chlamydomonas PNAS, March 24, 2009; 106(12): 4713 - 4718. [Abstract] [Full Text] [PDF]

				V. Rajagopalan, A. Subramanian, D. E. Wilkes, D. G. Pennock, and D. J. Asai Dynein-2 Affects the Regulation of Ciliary Length but Is Not Required for Ciliogenesis in Tetrahymena thermophila Mol. Biol. Cell, January 1, 2009; 20(2): 708 - 720. [Abstract] [Full Text] [PDF]

				M. Evangelista, T. Y. Lim, J. Lee, L. Parker, A. Ashique, A. S. Peterson, W. Ye, D. P. Davis, and F. J. de Sauvage Kinome siRNA Screen Identifies Regulators of Ciliogenesis and Hedgehog Signal Transduction Sci. Signal., September 30, 2008; 1(39): ra7 - ra7. [Abstract] [Full Text] [PDF]

				D. Wloga, I. Strzyzewska-Jowko, J. Gaertig, and M. Jerka-Dziadosz Septins Stabilize Mitochondria in Tetrahymena thermophila Eukaryot. Cell, August 1, 2008; 7(8): 1373 - 1386. [Abstract] [Full Text] [PDF]

				D. Wloga, K. Rogowski, N. Sharma, J. Van Dijk, C. Janke, B. Edde, M.-H. Bre, N. Levilliers, V. Redeker, J. Duan, et al. Glutamylation on {alpha}-Tubulin Is Not Essential but Affects the Assembly and Functions of a Subset of Microtubules in Tetrahymena thermophila Eukaryot. Cell, August 1, 2008; 7(8): 1362 - 1372. [Abstract] [Full Text] [PDF]

				E. Sohara, Y. Luo, J. Zhang, D. K. Manning, D. R. Beier, and J. Zhou Nek8 Regulates the Expression and Localization of Polycystin-1 and Polycystin-2 J. Am. Soc. Nephrol., March 1, 2008; 19(3): 469 - 476. [Abstract] [Full Text] [PDF]

				N. Sharma, J. Bryant, D. Wloga, R. Donaldson, R. C. Davis, M. Jerka-Dziadosz, and J. Gaertig Katanin regulates dynamics of microtubules and biogenesis of motile cilia J. Cell Biol., September 7, 2007; 178(6): 1065 - 1079. [Abstract] [Full Text] [PDF]

				L. A. Smith, N. O. Bukanov, H. Husson, R. J. Russo, T. C. Barry, A. L. Taylor, D. R. Beier, and O. Ibraghimov-Beskrovnaya Development of Polycystic Kidney Disease in Juvenile Cystic Kidney Mice: Insights into Pathogenesis, Ciliary Abnormalities, and Common Features with Human Disease J. Am. Soc. Nephrol., October 1, 2006; 17(10): 2821 - 2831. [Abstract] [Full Text] [PDF]