QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

Vol. 17, Issue 7, 2942-2951, July 2006

This Article Full Text Full Text (PDF) Supplemental Material All Versions of this Article: E05-12-1157v1 17/7/2942    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Collier, S. Articles by Shaw, P. Search for Related Content PubMed PubMed Citation Articles by Collier, S. Articles by Shaw, P.

A Distant Coilin Homologue Is Required for the Formation of Cajal Bodies in Arabidopsis

Sarah Collier^*,, Alison Pendle^*,, Kurt Boudonck^*,, Tjeerd van Rij^*,, Liam Dolan^*, and Peter Shaw^*

*John Innes Centre, Colney, Norwich NR4 7UH, United Kingdom; CIIT Centers for Health Research, Research Triangle Park, NC 27709-2137; and Institute of Biology, Leiden University, 2333 AL Leiden, The Netherlands

Submitted December 21, 2005; Revised April 11, 2006; Accepted April 12, 2006
Monitoring Editor: A. Gregory Matera

Cajal bodies (CBs) are subnuclear bodies that are widespread in eukaryotes, being found in mammals, many other vertebrates and in all plant species so far examined. They are mobile structures, moving, fusing, and budding within the nucleus. Here we describe a screen for Arabidopsis mutants with altered CBs and describe mutants that have smaller Cajal bodies (ncb-2, ncb-3), lack them altogether (ncb-1), have increased numbers of CBs (pcb) or have flattened CBs (ccb). We have identified the gene affected in the ncb mutants as a distant homolog of the vertebrate gene that encodes coilin (At1g13030) and have termed the resulting protein Atcoilin. A T-DNA insertional mutant in this gene (ncb-4) also lacks Cajal bodies. Overexpression of Atcoilin cDNA in ncb-1 restores Cajal bodies, which recruit U2B'' as in the wild type, but which are, however, much larger than in the wild type. Thus we have shown that At1g13030 is required for Cajal body formation in Arabidopsis, and we hypothesize that the level of its expression is correlated with Cajal body size. The Atcoilin gene is unaffected in pcb and ccb, suggesting that other genes can also affect CBs.

The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).

These authors contributed equally to this work.

Address correspondence to: Peter J. Shaw ( peter.shaw{at}bbsrc.ac.uk)

Abbreviations used: CB, Cajal body.

This article has been cited by other articles:

				Y. Fujioka, M. Utsumi, Y. Ohba, and Y. Watanabe Location of a Possible miRNA Processing Site in SmD3/SmB Nuclear Bodies in Arabidopsis Plant Cell Physiol., September 1, 2007; 48(9): 1243 - 1253. [Abstract] [Full Text] [PDF]

				E. Morency, M. Sabra, F. Catez, P. Texier, and P. Lomonte A novel cell response triggered by interphase centromere structural instability J. Cell Biol., June 21, 2007; 177(5): 757 - 768. [Abstract] [Full Text] [PDF]

				L. Song, M.-H. Han, J. Lesicka, and N. Fedoroff Arabidopsis primary microRNA processing proteins HYL1 and DCL1 define a nuclear body distinct from the Cajal body PNAS, March 27, 2007; 104(13): 5437 - 5442. [Abstract] [Full Text] [PDF]