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Originally published as MBC in Press, 10.1091/mbc.E06-01-0041 on May 10, 2006

Vol. 17, Issue 7, 3318-3328, July 2006

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Annexin A4 Self-Association Modulates General Membrane Protein Mobility in Living CellsFormula

Alen Piljic, and Carsten Schultz

Gene Expression Programme, European Molecular Biology Laboratory, 69117 Heidelberg, Germany

Submitted January 17, 2006; Revised April 27, 2006; Accepted April 28, 2006
Monitoring Editor: Jean Gruenberg

Annexins are Ca2+-regulated phospholipid-binding proteins whose function is only partially understood. Annexin A4 is a member of this family that is believed to be involved in exocytosis and regulation of epithelial Cl– secretion. In this work, fluorescent protein fusions of annexin A4 were used to investigate Ca2+-induced annexin A4 translocation and self-association on membrane surfaces in living cells. We designed a novel, genetically encoded, FRET sensor (CYNEX4) that allowed for easy quantification of translocation and self-association. Mobility of annexin A4 on membrane surfaces was investigated by FRAP. The experiments revealed the immobile nature of annexin A4 aggregates on membrane surfaces, which in turn strongly reduced the mobility of transmembrane and plasma membrane associated proteins. Our work provides mechanistic insight into how annexin A4 may regulate plasma membrane protein function.

This was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E06.01-0041) on May 10, 2006.

Formula The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).

Address correspondence to: Carsten Schultz ( schultz{at}embl.de)

Abbreviations used: [Ca2+]i, intracellular calcium concentration; CaMKII, multifunctional calcium/calmodulin-dependent protein kinase II; CaCC, calcium-activated chloride conductance; CF, cystic fibrosis; CYNEX4, cyan-yellow-labelled annexin A4; ECFP, enhanced cyan fluorescent protein; EYFP, enhanced yellow fluorescent protein; FRET, fluorescence resonance energy transfer; PLC{delta}1, phospholipase C{delta}1.




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