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Vol. 17, Issue 8, 3397-3408, August 2006

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Targeting of Transmembrane Protein Shrew-1 to Adherens Junctions Is Controlled by Cytoplasmic Sorting Motifs

Viktor Jakob^*, Alexander Schreiner^*, Ritva Tikkanen, and Anna Starzinski-Powitz^*

*Institute of Cell Biology and Neuroscience, Johann-Wolfgang Goethe University of Frankfurt, D-60323 Frankfurt am Main, Germany; and Institute of Biochemistry II, University Clinic of Frankfurt, D-60590 Frankfurt am Main, Germany

Submitted November 10, 2005; Revised May 3, 2006; Accepted May 5, 2006
Monitoring Editor: Jennifer Lippincott-Schwartz

We recently identified transmembrane protein shrew-1 and showed that it is able to target to adherens junctions in polarized epithelial cells. This suggested shrew-1 possesses specific basolateral sorting motifs, which we analyzed by mutational analysis. Systematic mutation of amino acids in putative sorting signals in the cytoplasmic domain of shrew-1 revealed three tyrosines and a dileucine motif necessary for basolateral sorting. Substitution of these amino acids leads to apical localization of shrew-1. By applying tannic acid to either the apical or basolateral part of polarized epithelial cells, thereby blocking vesicle fusion with the plasma membrane, we obtained evidence that the apically localized mutants were primarily targeted to the basolateral membrane and were then redistributed to the apical domain. Further support for a postendocytic sorting mechanism of shrew-1 was obtained by demonstrating that µ1B, a subunit of the epithelial cell-specific adaptor complex AP-1B, interacts with shrew-1. In conclusion, our data provide evidence for a scenario where shrew-1 is primarily delivered to the basolateral membrane by a so far unknown mechanism. Once there, adaptor protein complex AP-1B is involved in retaining shrew-1 at the basolateral membrane by postendocytic sorting mechanisms.

Address correspondence to: Anna Starzinski-Powitz ( starzinski-powitz{at}em.uni-frankfurt.de)

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