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Vol. 17, Issue 8, 3521-3533, August 2006
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Departments of *Neuroscience, Molecular, Microbial, and Structural Biology, and Biomedical Science Graduate Program, University of Connecticut Health Center, Farmington, CT 06030
Submitted October 12, 2005;
Revised May 15, 2006;
Accepted June 1, 2006
Monitoring Editor: Karsten Weis
Heterogeneous nuclear ribonucleoprotein (hnRNP) A2 is a trans-acting RNA-binding protein that mediates trafficking of RNAs containing the cis-acting A2 response element (A2RE). Previous work has shown that A2RE RNAs are transported to myelin in oligodendrocytes and to dendrites in neurons. hnRNP E1 is an RNA-binding protein that regulates translation of specific mRNAs. Here, we show by yeast two-hybrid analysis, in vivo and in vitro coimmunoprecipitation, in vitro cross-linking, and fluorescence correlation spectroscopy that hnRNP E1 binds to hnRNP A2 and is recruited to A2RE RNA in an hnRNP A2-dependent manner. hnRNP E1 is colocalized with hnRNP A2 and A2RE mRNA in granules in dendrites of oligodendrocytes. Overexpression of hnRNP E1 or microinjection of exogenous hnRNP E1 in neural cells inhibits translation of A2RE mRNA, but not of non-A2RE RNA. Excess hnRNP E1 added to an in vitro translation system reduces translation efficiency of A2RE mRNA, but not of nonA2RE RNA, in an hnRNP A2-dependent manner. These results are consistent with a model where hnRNP E1 recruited to A2RE RNA granules by binding to hnRNP A2 inhibits translation of A2RE RNA during granule transport.
Address correspondence to: Elisa Barbarese ( barbarese{at}nso2.uchc.edu)
Abbreviations used: A2RE, A2 response element; CSK, cytoskeleton; DICE, differentiation control element; FCS, fluorescence correlation spectroscopy; GFP, green fluorescent protein; hnRNP, heterogeneous nuclear ribonucleoprotein; KH, hnRNP K homology; MBP, myelin basic protein; RRL, rabbit reticulocyte lysate.
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