Molecular Biology of the Cell click for CBE Life Science Education Page

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
 QUICK SEARCH:   [advanced]


     


Originally published as MBC in Press, 10.1091/mbc.E06-03-0240 on June 7, 2006

Vol. 17, Issue 8, 3705-3716, August 2006

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Material
Right arrow All Versions of this Article:
E06-03-0240v1
17/8/3705    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Qi, W.
Right arrow Articles by Yu, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Qi, W.
Right arrow Articles by Yu, H.

Phosphorylation- and Polo-Box–dependent Binding of Plk1 to Bub1 Is Required for the Kinetochore Localization of Plk1Formula

Wei Qi, Zhanyun Tang, and Hongtao Yu

Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, TX 75390-9041

Submitted March 27, 2006; Accepted May 31, 2006
Monitoring Editor: Mark Solomon

Polo-like kinase 1 (Plk1) is required for the generation of the tension-sensing 3F3/2 kinetochore epitope and facilitates kinetochore localization of Mad2 and other spindle checkpoint proteins. Here, we investigate the mechanism by which Plk1 itself is recruited to kinetochores. We show that Plk1 binds to budding uninhibited by benzimidazole 1 (Bub1) in mitotic human cells. The Plk1–Bub1 interaction requires the polo-box domain (PBD) of Plk1 and is enhanced by cyclin-dependent kinase 1 (Cdk1)-mediated phosphorylation of Bub1 at T609. The PBD-dependent binding of Plk1 to Bub1 facilitates phosphorylation of Bub1 by Plk1 in vitro. Depletion of Bub1 in HeLa cells by RNA interference (RNAi) diminishes the kinetochore localization of Plk1. Ectopic expression of the wild-type Bub1, but not the Bub1-T609A mutant, in Bub1-RNAi cells restores the kinetochore localization of Plk1. Our results suggest that phosphorylation of Bub1 at T609 by Cdk1 creates a docking site for the PBD of Plk1 and facilitates the kinetochore recruitment of Plk1.


This was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E06-03-0240) on June 7, 2006.

Formula The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).

Address correspondence to: Hongtao Yu ( hongtao.yu{at}utsouthwestern.edu)

Abbreviations used: PBD, polo-box domain; Plk1, Polo-like kinase 1; Bub1, budding uninhibited by benzimidazole 1; BubR1, Bub1-related protein; Cdk1, cyclin-dependent kinase 1; INCENP, inner centromere protein; IP, immunoprecipitation; RNAi, RNA interference.




This article has been cited by other articles:


Home page
J. Cell Sci.Home page
X. Zhang, Q. Chen, J. Feng, J. Hou, F. Yang, J. Liu, Q. Jiang, and C. Zhang
Sequential phosphorylation of Nedd1 by Cdk1 and Plk1 is required for targeting of the {gamma}TuRC to the centrosome
J. Cell Sci., July 1, 2009; 122(13): 2240 - 2251.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
J. Kang and H. Yu
Kinase Signaling in the Spindle Checkpoint
J. Biol. Chem., June 5, 2009; 284(23): 15359 - 15363.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. Biol.Home page
K. Kishi, M. A. T. M. van Vugt, K.-i. Okamoto, Y. Hayashi, and M. B. Yaffe
Functional Dynamics of Polo-Like Kinase 1 at the Centrosome
Mol. Cell. Biol., June 1, 2009; 29(11): 3134 - 3150.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
J. Fu, M. Bian, J. Liu, Q. Jiang, and C. Zhang
A single amino acid change converts Aurora-A into Aurora-B-like kinase in terms of partner specificity and cellular function
PNAS, April 28, 2009; 106(17): 6939 - 6944.
[Abstract] [Full Text] [PDF]


Home page
JCBHome page
A. Lindqvist, V. Rodriguez-Bravo, and R. H. Medema
The decision to enter mitosis: feedback and redundancy in the mitotic entry network
J. Cell Biol., April 20, 2009; 185(2): 193 - 202.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
N. Watanabe, T. Sekine, M. Takagi, J.-i. Iwasaki, N. Imamoto, H. Kawasaki, and H. Osada
Deficiency in Chromosome Congression by the Inhibition of Plk1 Polo Box Domain-dependent Recognition
J. Biol. Chem., January 23, 2009; 284(4): 2344 - 2353.
[Abstract] [Full Text] [PDF]


Home page
Eukaryot CellHome page
T. Umeyama and C. C. Wang
Polo-Like Kinase Is Expressed in S/G2/M Phase and Associated with the Flagellum Attachment Zone in both Procyclic and Bloodstream Forms of Trypanosoma brucei
Eukaryot. Cell, September 1, 2008; 7(9): 1582 - 1590.
[Abstract] [Full Text] [PDF]


Home page
GeneticsHome page
M. Lynch, A. Seyfert, B. Eads, and E. Williams
Localization of the Genetic Determinants of Meiosis Suppression in Daphnia pulex
Genetics, September 1, 2008; 180(1): 317 - 327.
[Abstract] [Full Text] [PDF]


Home page
DevelopmentHome page
Y. Nishi, E. Rogers, S. M. Robertson, and R. Lin
Polo kinases regulate C. elegans embryonic polarity via binding to DYRK2-primed MEX-5 and MEX-6
Development, February 15, 2008; 135(4): 687 - 697.
[Abstract] [Full Text] [PDF]


Home page
Mol. Biol. CellHome page
A. M. Bentley, G. Normand, J. Hoyt, and R. W. King
Distinct Sequence Elements of Cyclin B1 Promote Localization to Chromatin, Centrosomes, and Kinetochores during Mitosis
Mol. Biol. Cell, December 1, 2007; 18(12): 4847 - 4858.
[Abstract] [Full Text] [PDF]


Home page
Mol. Biol. CellHome page
A. Santamaria, R. Neef, U. Eberspacher, K. Eis, M. Husemann, D. Mumberg, S. Prechtl, V. Schulze, G. Siemeister, L. Wortmann, et al.
Use of the Novel Plk1 Inhibitor ZK-Thiazolidinone to Elucidate Functions of Plk1 in Early and Late Stages of Mitosis
Mol. Biol. Cell, October 1, 2007; 18(10): 4024 - 4036.
[Abstract] [Full Text] [PDF]


Home page
Genes Dev.Home page
S. Elowe, S. Hummer, A. Uldschmid, X. Li, and E. A. Nigg
Tension-sensitive Plk1 phosphorylation on BubR1 regulates the stability of kinetochore microtubule interactions
Genes & Dev., September 1, 2007; 21(17): 2205 - 2219.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
S. Matsumura, F. Toyoshima, and E. Nishida
Polo-like Kinase 1 Facilitates Chromosome Alignment during Prometaphase through BubR1
J. Biol. Chem., May 18, 2007; 282(20): 15217 - 15227.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
W. Qi and H. Yu
KEN-Box-dependent Degradation of the Bub1 Spindle Checkpoint Kinase by the Anaphase-promoting Complex/Cyclosome
J. Biol. Chem., February 9, 2007; 282(6): 3672 - 3679.
[Abstract] [Full Text] [PDF]




Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Copyright © 2006 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.