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Vol. 18, Issue 11, 4304-4316, November 2007
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*Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Ibaraki 305-8566, Japan; Graduate School of Life and Environmental Science, University of Tsukuba, Ibaraki 305-8572, Japan; and Center for Tsukuba Advanced Research Alliance, Graduate School of Life and Environmental Science, University of Tsukuba, Ibaraki 305-8577, Japan
Submitted May 22, 2007;
Revised August 9, 2007;
Accepted August 17, 2007
Monitoring Editor: Sean Munro
The glycosylphosphatidylinositol (GPI)-anchored proteins are subjected to lipid remodeling during their biosynthesis. In the yeast Saccharomyces cerevisiae, the mature GPI-anchored proteins contain mainly ceramide or diacylglycerol with a saturated long-fatty acid, whereas conventional phosphatidylinositol (PI) used for GPI biosynthesis contains an unsaturated fatty acid. Here, we report that S. cerevisiae Cwh43p, whose N-terminal region contains a sequence homologous to mammalian PGAP2, is involved in the remodeling of the lipid moiety of GPI anchors to ceramides. In cwh43 disruptant cells, the PI moiety of the GPI-anchored protein contains a saturated long fatty acid and lyso-PI but not inositolphosphorylceramides, which are the main lipid moieties of GPI-anchored proteins from wild-type cells. Moreover, the C-terminal region of Cwh43p (Cwh43-C), which is not present in PGAP2, is essential for the ability to remodel GPI lipids to ceramides. The N-terminal region of Cwh43p (Cwh43-N) is associated with Cwh43-C, and it enhanced the lipid remodeling to ceramides by Cwh43-C. Our results also indicate that mouse FRAG1 and C130090K23, which are homologous to Cwh43-N and -C, respectively, share these activities.
The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).
Address correspondence to: Yoshifumi Jigami (jigami.yoshi{at}aist.go.jp)
Abbreviations used: CFW, calcofluor white; Con A, concanavalin A; DRM, detergent-resistant membrane; DHS, dihydrosphingosine; ER, endoplasmic reticulum; IPC, inositolphosphorylceramide; IPC/B, IPC consisting of phytosphingosine and a C26:0 fatty acid; IPC/C, IPC consisting of phytosphingosine and a hydroxylated C26:0 fatty acid; mRFP, monomeric red fluorescent protein; PAGE, polyacrylamide gel electrophoresis; pG1, phosphatidylinositol with a C26:0 fatty acid in sn-2 position; PI, phosphatidylinositol; TLC, thin layer chromatography.
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