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Vol. 18, Issue 12, 5124-5138, December 2007

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Identification of an Intramolecular Interaction Important for the Regulation of GIT1 Functions

Cell Adhesion Unit, Dibit, San Raffaele Scientific Institute, 20132 Milan, Italy

Submitted June 11, 2007; Revised September 7, 2007; Accepted September 13, 2007
Monitoring Editor: Josephine Adams

G-protein coupled receptor kinase-interacting protein (GIT) proteins include an N-terminal Arf GTPase-activating protein domain, and a C terminus that binds proteins regulating adhesion and motility. Given their ability to form large molecular assemblies, the GIT1 protein must be tightly regulated. However, the mechanisms regulating GIT1 functions are poorly characterized. We found that carboxy-terminal–truncated fragments of GIT1 bind their partners with higher efficiency compared with the full-length GIT1. We have explored the hypothesis that GIT1 is regulated by an intramolecular mechanism, and we identified two distinct intramolecular interactions between the N and C terminus of GIT1. The release of these interactions increases binding of GIT1 to paxillin and liprin-, and it correlates with effects on cell spreading. Analysis of cells plated on fibronectin has shown that different deletion mutants of GIT1 either enhance or inhibit spreading, depending on their subcellular localization. Moreover, although the association between βPIX and GIT1 is insufficient to activate GIT1 binding to paxillin, binding of a PAK1 fragment including the βPIX-binding domain enhances paxillin binding to βPIX/GIT1, indicating that p21-activated kinase can activate the binding of paxillin to GIT1 by a kinase-independent mechanism. The release of the identified intramolecular interaction seems to be an important mechanism for the regulation of GIT1 functions.

The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).

Address correspondence to: Ivan de Curtis (decurtis.ivan{at}hsr.it).

Abbreviations used: βPIX, p21-activated kinase interacting exchange factor; ArfGAP, Arf GTPase-activating protein; GIT, G-protein coupled receptor kinase-interacting protein; PBS, paxillin-binding site; SHD, Spa2-homology domain.