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Vol. 18, Issue 3, 1018-1029, March 2007
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*University of Pittsburgh Cancer Institute, Pittsburgh, PA 15312; Department of Cell Biology and Physiology, University of Pittsburgh, School of Medicine, Pittsburgh, PA 15261; Weill Medical College of Cornell University, New York, NY 10021; and Neurological Sciences Institute, Oregon Health and Science University, Beaverton, OR 97006
Submitted September 11, 2006;
Revised December 13, 2006;
Accepted January 2, 2007
Monitoring Editor: Mark Solomon
Lens development requires the precise coordination of cell division and differentiation. The mechanisms by which the differentiation program is initiated after cell cycle arrest remains not well understood. Cyclin-dependent kinase inhibitors (CKIs), such as p15 and p21, have been suggested to be critical components that inhibit G1 progression and therefore, their activation is necessary for quiescence and important for the onset of differentiation. Regulation of p15 and p21 is principally governed by transforming growth factor (TGF)-
–signaling pathway. We have identified that Cdh1/APC, a critical ubiquitin protein ligase, plays an important role in regulating lens differentiation by facilitating TGF-–induced degradation of SnoN, a transcriptional corepressor that needs to be removed for transcriptional activation of p15 and p21. The depletion of Cdh1 by RNA interference attenuates the TGF-–mediated induction of p15 and p21 and significantly blocks lens differentiation. Expression of nondegradable SnoN also noticeably attenuates lens induction. Furthermore, we have shown that Cdh1 and SnoN form a complex at the onset of lens differentiation. In vivo histological analysis confirms our biochemical and genetic results. Thus, Cdh1/APC is crucial to the coordination of cell cycle progression and the initiation of lens differentiation through mediating TGF-–signaling-induced destruction of SnoN.
The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).
Address correspondence to: Yong Wan (yow4{at}pitt.edu)
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