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Originally published as MBC in Press, 10.1091/mbc.E06-10-0974 on January 10, 2007

Vol. 18, Issue 3, 1056-1063, March 2007

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VAMP8/Endobrevin as a General Vesicular SNARE for Regulated Exocytosis of the Exocrine System

Cheng-Chun Wang*, Hong Shi*, Ke Guo*, Chee Peng Ng*, Jie Li*, Bin Qi Gan*, Hwee Chien Liew*, Jukka Leinonen{dagger}, Hannu Rajaniemi{dagger}, Zhi Hong Zhou*, Qi Zeng*, and Wanjin Hong*

*Institute of Molecular and Cell Biology, Singapore 138673, Singapore; and {dagger}Department of Anatomy and Cell Biology, University of Oulu, 90014 Oulu, Finland

Submitted November 2, 2006; Revised December 21, 2006; Accepted January 2, 2007
Monitoring Editor: Vivek Malhotra

The molecular mechanism governing the regulated secretion of most exocrine tissues remains elusive, although VAMP8/endobrevin has recently been shown to be the major vesicular SNARE (v-SNARE) of zymogen granules of pancreatic exocrine acinar cells. In this article, we have characterized the role of VAMP8 in the entire exocrine system. Immunohistochemical studies showed that VAMP8 is expressed in all examined exocrine tissues such as salivary glands, lacrimal (tear) glands, sweat glands, sebaceous glands, mammary glands, and the prostate. Severe anomalies were observed in the salivary and lacrimal glands of VAMP8-null mice. Mutant salivary glands accumulated amylase and carbonic anhydrase VI. Electron microscopy revealed an accumulation of secretory granules in the acinar cells of mutant parotid and lacrimal glands. Pilocarpine-stimulated secretion of saliva proteins was compromised in the absence of VAMP8. Protein aggregates were observed in mutant lacrimal glands. VAMP8 may interact with syntaxin 4 and SNAP-23. These results suggest that VAMP8 may act as a v-SNARE for regulated secretion of the entire exocrine system.

This was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E06-10-0974) on January 10, 2007.

Address correspondence to: Wanjin Hong (mcbhwj{at}imcb.a-star.edu.sg)




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