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Originally published as MBC in Press, 10.1091/mbc.E06-08-0683 on December 27, 2006

Vol. 18, Issue 3, 919-929, March 2007

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Atg19 Mediates a Dual Interaction Cargo Sorting Mechanism in Selective AutophagyFormula

Chiung-Ying Chang*, and Wei-Pang Huang*,{dagger}

*Institute of Zoology and {dagger}Department of Life Science, National Taiwan University, Taipei 106, Taiwan, Republic of China

Submitted August 7, 2006; Revised November 27, 2006; Accepted December 20, 2006
Monitoring Editor: Suresh Subramani

Autophagy is a catabolic membrane-trafficking mechanism conserved in all eukaryotic cells. In addition to the nonselective transport of bulk cytosol, autophagy is responsible for efficient delivery of the vacuolar enzyme Ape1 precursor (prApe1) in the budding yeast Saccharomyces cerevisiae, suggesting the presence of a prApe1 sorting machinery. Sequential interactions between Atg19-Atg11 and Atg19-Atg8 pairs are thought responsible for targeting prApe1 to the vesicle formation site, the preautophagosomal structure (PAS), and loading it into transport vesicles, respectively. However, the different patterns of prApe1 transport defect seen in the atg11{Delta} and atg19{Delta} strains seem to be incompatible with this model. Here we report that prApe1 could not be targeted to the PAS and failed to be delivered into the vacuole in atg8{Delta} atg11{Delta} double knockout cells regardless of the nutrient conditions. We postulate that Atg19 mediates a dual interaction prApe1-sorting mechanism through independent, instead of sequential, interactions with Atg11 and Atg8. In addition, to efficiently deliver prApe1 to the vacuole, a proper interaction between Atg11 and Atg9 is indispensable. We speculate that Atg11 may elicit a cargo-loading signal and induce Atg9 shuttling to a specific PAS site, where Atg9 relays the signal and recruits other Atg proteins to induce vesicle formation.

This was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E06-08-0683) on December 27, 2006.

Formula The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).

Address correspondence to: Wei-Pang Huang (wphuang{at}ntu.edu.tw)




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