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Originally published as MBC in Press, 10.1091/mbc.E06-08-0741 on March 7, 2007

Vol. 18, Issue 5, 1816-1825, May 2007

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A Ser/Thr Kinase Required for Membrane-associated Assembly of the Major Sperm Protein Motility Apparatus in the Amoeboid Sperm of Ascaris

Kexi Yi*, Shawnna M. Buttery*,{dagger}, Murray Stewart{ddagger}, and Thomas M. Roberts*

*Department of Biological Science, Florida State University, Tallahassee, FL 32306; and {ddagger}Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 2QH, United Kingdom

Submitted August 24, 2006; Revised January 29, 2007; Accepted February 26, 2007
Monitoring Editor: Yu-li Wang

Leading edge protrusion in the amoeboid sperm of Ascaris suum is driven by the localized assembly of the major sperm protein (MSP) cytoskeleton in the same way that actin assembly powers protrusion in other types of crawling cell. Reconstitution of this process in vitro led to the identification of two accessory proteins required for MSP polymerization: an integral membrane phosphoprotein, MSP polymerization–organizing protein (MPOP), and a cytosolic component, MSP fiber protein 2 (MFP2). Here, we identify and characterize a 34-kDa cytosolic protein, MSP polymerization–activating kinase (MPAK) that links the activities of MPOP and MFP2. Depletion/add-back assays of sperm extracts showed that MPAK, which is a member of the casein kinase 1 family of Ser/Thr protein kinases, is required for motility. MPOP and MPAK comigrated by native gel electrophoresis, coimmunoprecipitated, and colocalized by immunofluorescence, indicating that MPOP binds to and recruits MPAK to the membrane surface. MPAK, in turn, phosphorylated MFP2 on threonine residues, resulting in incorporation of MFP2 into the cytoskeleton. Beads coated with MPAK assembled a surrounding cloud of MSP filaments when incubated in MPAK-depleted sperm extract, but only when supplemented with detergent-solubilized MPOP. Our results suggest that interactions involving MPOP, MPAK, and MFP2 focus MSP polymerization to the plasma membrane at the leading edge of the cell thereby generating protrusion and minimizing nonproductive filament formation elsewhere.

This article was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E06-08-0741) on March 7, 2007.

{dagger} Present address: Dana Farber Cancer Institute, Boston, MA 02115.

Address correspondence to: Thomas M. Roberts (roberts{at}bio.fsu.edu).




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K. Yi, X. Wang, M. R. Emmett, A. G. Marshall, M. Stewart, and T. M. Roberts
Dephosphorylation of Major Sperm Protein (MSP) Fiber Protein 3 by Protein Phosphatase 2A during Cell Body Retraction in the MSP-based Amoeboid Motility of Ascaris Sperm
Mol. Biol. Cell, July 15, 2009; 20(14): 3200 - 3208.
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