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Originally published as MBC in Press, 10.1091/mbc.E06-09-0823 on March 7, 2007

Vol. 18, Issue 5, 1943-1952, May 2007

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Slug Is Required for Cell Survival during Partial Epithelial-Mesenchymal Transition of HGF-induced TubulogenesisFormula

Pascale Leroy, and Keith E. Mostov

Department of Anatomy, University of California, San Francisco, CA 94158-2517

Submitted September 18, 2006; Revised February 6, 2007; Accepted February 26, 2007
Monitoring Editor: Ben Margolis

Transcription factors of the Snail family are key regulators of epithelial-mesenchymal transition (EMT). In many processes during development or disease, cells do not acquire all the characteristics associated with EMT, leading to what we refer to as partial EMT (p-EMT). However, little is known of the implications of the Snail transcription factors in processes that only involve a p-EMT. To assess this, we used the hepatocyte growth factor (HGF)-induced Madin-Darby canine kidney tubulogenesis system, which provides a three-dimensional culture model of a morphogenetic process including a p-EMT. We found that although Slug (Snail2) is highly and transitory up-regulated during the p-EMT phase of tubulogenesis, it is not a repressor of E-cadherin during this process. Using inducible knockdown of Slug, we demonstrate that Slug is not an inducer of cell movement and instead is required for survival during p-EMT. We conclude that in epithelial cells, promoting cell survival can be a primary function of Slug, rather than being acquired concomitantly with EMT.

This was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E06-09-0823) on March 7, 2007.

Formula The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).

Address correspondence to: Pascale Leroy (pascale.leroy{at}ucsf.edu)

Abbreviations used: EMT, epithelial-mesenchymal transition; HGF, hepatocyte growth factor; p-EMT, partial epithelial-mesenchymal transition.




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W.-Y. Huang, Z.-G. Li, H. Rus, X. Wang, P. A. Jose, and S.-Y. Chen
RGC-32 Mediates Transforming Growth Factor-{beta}-induced Epithelial-Mesenchymal Transition in Human Renal Proximal Tubular Cells
J. Biol. Chem., April 3, 2009; 284(14): 9426 - 9432.
[Abstract] [Full Text] [PDF]


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