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Vol. 18, Issue 6, 2013-2025, June 2007
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*Department of Cell Biology, University of Virginia, Charlottesville, VA 22908-0732;
Institut National de la Santé et de la Recherche Médicale, U837, 59045 Lille, France;
Université Lille 2, Faculté de Médecine, Institut de Médecine Prédictive et Recherche Thérapeutique, Jean-Pierre Aubert Research Center, 59045 Lille, France; and
The Rockefeller University, New York, NY 10021
Submitted April 24, 2006;
Revised March 6, 2007;
Accepted March 20, 2007
Monitoring Editor: Jean Schwarzbauer
E-cadherin function leads to the density-dependent contact inhibition of cell growth. Because cadherins control the overall state of cell contact, cytoskeletal organization, and the establishment of many other kinds of cell interactions, it remains unknown whether E-cadherin directly transduces growth inhibitory signals. To address this question, we have selectively formed E-cadherin homophilic bonds at the cell surface of isolated epithelial cells by using functionally active recombinant E-cadherin protein attached to microspheres. We find that E-cadherin ligation alone reduces the frequency of cells entering the S phase, demonstrating that E-cadherin ligation directly transduces growth inhibitory signals. E-cadherin binding to
-catenin is required for cell growth inhibition, but
-catenin/T-cell factor transcriptional activity is not involved in growth inhibition resulting from homophilic binding. Neither E-cadherin binding to p120-catenin nor
-catenin binding to
-catenin, and thereby the actin cytoskeleton, is required for growth inhibition. E-cadherin ligation also inhibits epidermal growth factor (EGF) receptor-mediated growth signaling by a
-catenindependent mechanism. It does not affect EGF receptor autophosphorylation or activation of ERK, but it inhibits transphosphorylation of Tyr845 and activation of signal transducers and activators of transcription 5. Thus, E-cadherin homophilic binding independent of other cell contacts directly transduces growth inhibition by a
-catenindependent mechanism that inhibits selective signaling functions of growth factor receptors.
The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).
Address correspondence to: Barry M. Gumbiner (gumbiner{at}virginia.edu).
Abbreviations used: BrdU, 5-bromodeoxyuridine; EGF, epidermal growth factor; EGFR, epidermal growth factor receptor; ERK, extracellular signal-regulated kinase; Fc-hE, Fc-hEcadherin; FL, full length; LEF, leukocyte enhancer factor; siRNA, small interfering RNA; STAT, signal transducers and activators of transcription; TCF, T cell factor.
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