Molecular Biology of the Cell click for CBE Life Science Education Page

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

Originally published as MBC in Press, 10.1091/mbc.E06-08-0695 on March 21, 2007 Originally published as MBC in Press, 10.1091/mbc.E06-08-0695 on March 14, 2007

Vol. 18, Issue 6, 2081-2089, June 2007

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Material
Right arrow All Versions of this Article:
E06-08-0695v1
E06-08-0695v2
18/6/2081    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Haghnia, M.
Right arrow Articles by Goldstein, L. S.B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Haghnia, M.
Right arrow Articles by Goldstein, L. S.B.

Dynactin Is Required for Coordinated Bidirectional Motility, but Not for Dynein Membrane AttachmentFormula Formula

Marjan Haghnia*,{dagger}, Valeria Cavalli*,{dagger}, Sameer B. Shah*, Kristina Schimmelpfeng*, Richard Brusch*, Ge Yang{ddagger}, Cheryl Herrera*, Aaron Pilling§, and Lawrence S.B. Goldstein*

*Howard Hughes Medical Institute and Department of Cellular and Molecular Medicine, School of Medicine, University of California, San Diego, La Jolla, CA 92093-0683; {ddagger}Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037; and §Department of Physiology, University of Pennsylvania, Philadelphia, PA 19104

Submitted August 11, 2006; Revised February 9, 2007; Accepted March 2, 2007
Monitoring Editor: Yixian Zheng

Transport of cellular and neuronal vesicles, organelles, and other particles along microtubules requires the molecular motor protein dynein (Mallik and Gross, 2004). Critical to dynein function is dynactin, a multiprotein complex commonly thought to be required for dynein attachment to membrane compartments (Karki and Holzbaur, 1999). Recent work also has found that mutations in dynactin can cause the human motor neuron disease amyotrophic lateral sclerosis (Puls et al., 2003). Thus, it is essential to understand the in vivo function of dynactin. To test directly and rigorously the hypothesis that dynactin is required to attach dynein to membranes, we used both a Drosophila mutant and RNA interference to generate organisms and cells lacking the critical dynactin subunit, actin-related protein 1. Contrary to expectation, we found that apparently normal amounts of dynein associate with membrane compartments in the absence of a fully assembled dynactin complex. In addition, anterograde and retrograde organelle movement in dynactin deficient axons was completely disrupted, resulting in substantial changes in vesicle kinematic properties. Although effects on retrograde transport are predicted by the proposed function of dynactin as a regulator of dynein processivity, the additional effects we observed on anterograde transport also suggest potential roles for dynactin in mediating kinesin-driven transport and in coordinating the activity of opposing motors (King and Schroer, 2000).

This article was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E06-08-0695) on March 14, 2007.

Formula Formula The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).

{dagger} These authors contributed equally to this work.

Address correspondence to: Lawrence S.B. Goldstein (lgoldstein{at}ucsd.edu).

Abbreviations used: Arp1, actin-related protein 1; DHC, dynein heavy chain; KHC, kinesin heavy chain; p50, dynamitin; PNS, postnuclear supernatant; HSP, high-speed pellet; HSS, high-speed supernatant.




This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
K. J. Palmer, H. Hughes, and D. J. Stephens
Specificity of Cytoplasmic Dynein Subunits in Discrete Membrane-trafficking Steps
Mol. Biol. Cell, June 15, 2009; 20(12): 2885 - 2899.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. R. Kardon, S. L. Reck-Peterson, and R. D. Vale
Regulation of the processivity and intracellular localization of Saccharomyces cerevisiae dynein by dynactin
PNAS, April 7, 2009; 106(14): 5669 - 5674.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
W.-H. Lien, V. I. Gelfand, and V. Vasioukhin
{alpha}-E-catenin binds to dynamitin and regulates dynactin-mediated intracellular traffic
J. Cell Biol., December 15, 2008; 183(6): 989 - 997.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. J. P. Iyadurai, J. T. Robinson, L. Ma, Y. He, S. Mische, M.-g. Li, W. Brown, A. Guichard, E. Bier, and T. S. Hays
Dynein and Star interact in EGFR signaling and ligand trafficking
J. Cell Sci., August 15, 2008; 121(16): 2643 - 2651.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Y. Shim, B. A. Samuels, J. Wang, G. Neumayer, C. Belzil, R. Ayala, Y. Shi, Y. Shi, L.-H. Tsai, and M. D. Nguyen
Ndel1 Controls the Dynein-mediated Transport of Vimentin during Neurite Outgrowth
J. Biol. Chem., May 2, 2008; 283(18): 12232 - 12240.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
F. M. Laird, M. H. Farah, S. Ackerley, A. Hoke, N. Maragakis, J. D. Rothstein, J. Griffin, D. L. Price, L. J. Martin, and P. C. Wong
Motor Neuron Disease Occurring in a Mutant Dynactin Mouse Model Is Characterized by Defects in Vesicular Trafficking
J. Neurosci., February 27, 2008; 28(9): 1997 - 2005.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Copyright © 2007 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.