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Vol. 18, Issue 7, 2707-2715, July 2007

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Exogenous MAL Reroutes Selected Hepatic Apical Proteins into the Direct Pathway in WIF-B Cells

Sai Prasad Ramnarayanan^*, Christina A. Cheng^*, Maria Bastaki, and Pamela L. Tuma^*

*Department of Biology, The Catholic University of America, Washington, DC 20064; and Graduate Environmental Studies Unit, The Evergreen State College, Olympia, WA 98505

Submitted February 2, 2007; Revised April 2, 2007; Accepted May 1, 2007
Monitoring Editor: Robert Parton

Unlike simple epithelial cells that directly target newly synthesized glycophosphatidylinositol (GPI)-anchored and single transmembrane domain (TMD) proteins from the trans-Golgi network to the apical membrane, hepatocytes use an indirect pathway: proteins are delivered to the basolateral domain and then selectively internalized and transcytosed to the apical plasma membrane. Myelin and lymphocyte protein (MAL) and MAL2 have been identified as regulators of direct and indirect apical delivery, respectively. Hepatocytes lack endogenous MAL consistent with the absence of direct apical targeting. Does MAL expression reroute hepatic apical residents into the direct pathway? We found that MAL expression in WIF-B cells induced the formation of cholesterol and glycosphingolipid-enriched Golgi domains that contained GPI-anchored and single TMD apical proteins; polymeric IgA receptor (pIgA-R), polytopic apical, and basolateral resident distributions were excluded. Basolateral delivery of newly synthesized apical residents was decreased in MAL-expressing cells concomitant with increased apical delivery; pIgA-R and basolateral resident delivery was unchanged. These data suggest that MAL rerouted selected hepatic apical proteins into the direct pathway.

The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).

Address correspondence to: Pamela L. Tuma (tuma{at}cua.edu)

Abbreviations used: 5'NT, 5'nucleotidase; APN, aminopeptidase N; BC, bile canaliculus; BFA, brefeldin A; CHX, cycloheximide; DPP IV, dipeptidyl peptidase IV; GPI, glycophosphatidylinositol; HA, hemagglutinin; MAL, myelin and lymphocyte protein; mCD, methyl--cyclodextrin; MRP2, multidrug resistance-associated protein 2; pIgA-R, polymeric IgA receptor; PM, plasma membrane; SAC, subapical compartment; TMD, transmembrane domain.