Molecular Biology of the Cell click for CBE Life Science Education Page

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

Originally published as MBC in Press, 10.1091/mbc.E07-03-0242 on July 5, 2007

Vol. 18, Issue 9, 3440-3450, September 2007

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Materials
Right arrow All Versions of this Article:
E07-03-0242v1
18/9/3440    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nelson, S. A.
Right arrow Articles by Cooper, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nelson, S. A.
Right arrow Articles by Cooper, J. A.

A Novel Pathway that Coordinates Mitotic Exit with Spindle PositionFormula Formula

Scott A. Nelson, and John A. Cooper

Department of Cell Biology, Washington University in St. Louis, St. Louis, MO 63110

Submitted March 14, 2007; Accepted June 26, 2007
Monitoring Editor: Kerry Bloom

In budding yeast, the spindle position checkpoint (SPC) delays mitotic exit until the mitotic spindle moves into the neck between the mother and bud. This checkpoint works by inhibiting the mitotic exit network (MEN), a signaling cascade initiated and controlled by Tem1, a small GTPase. Tem1 is regulated by a putative guanine exchange factor, Lte1, but the function and regulation of Lte1 remains poorly understood. Here, we identify novel components of the checkpoint that operate upstream of Lte1. We present genetic evidence in agreement with existing biochemical evidence for the molecular mechanism of a pathway that links microtubule-cortex interactions with Lte1 and mitotic exit. Each component of this pathway is required for the spindle position checkpoint to delay mitotic exit until the spindle is positioned correctly.

This was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E07-03-0242) on July 5, 2007.

Formula Formula The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).

Address correspondence to: John Cooper (jcooper{at}wustl.edu).

Abbreviations used: SPC, spindle position checkpoint; MEN, mitotic exit network; GAP, GTPase-activating protein; GEF, guanine exchange factor; SPB, spindle pole body; FEAR, cdc14 early anaphase release; snRNP, small nuclear ribonucleoprotein; FLIP, fluorescence loss in photobleaching.




This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
N. Delgehyr, C. S. J. Lopes, C. A. Moir, S. M. Huisman, and M. Segal
Dissecting the involvement of formins in Bud6p-mediated cortical capture of microtubules in S. cerevisiae
J. Cell Sci., November 15, 2008; 121(22): 3803 - 3814.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
J. Kim, S. S. Jang, and K. Song
Different Levels of Bfa1/Bub2 GAP Activity Are Required to Prevent Mitotic Exit of Budding Yeast Depending on the Type of Perturbations
Mol. Biol. Cell, October 1, 2008; 19(10): 4328 - 4340.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Copyright © 2007 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.