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Originally published as MBC in Press, 10.1091/mbc.E07-03-0258 on July 5, 2007

Vol. 18, Issue 9, 3472-3485, September 2007

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Pga1 Is an Essential Component of Glycosylphosphatidylinositol-Mannosyltransferase II of Saccharomyces cerevisiae

Keisuke Sato, Yoichi Noda, and Koji Yoda

Department of Biotechnology, University of Tokyo, Tokyo 113-8657, Japan

Submitted March 19, 2007; Revised June 21, 2007; Accepted June 22, 2007
Monitoring Editor: Akihiko Nakano

The Saccharomyces cerevisiae essential gene YNL158w/PGA1 encodes an endoplasmic reticulum (ER)-localized membrane protein. We constructed temperature-sensitive alleles of PGA1 by error-prone polymerase chain reaction mutagenesis to explore its biological role. Pulse-chase experiments revealed that the pga1ts mutants accumulated the ER-form precursor of Gas1 protein at the restrictive temperature. Transport of invertase and carboxypeptidase Y were not affected. Triton X-114 phase separation and [3H]inositol labeling indicated that the glycosylphosphatidylinositol (GPI)-anchoring was defective in the pga1ts mutants, suggesting that Pga1 is involved in GPI synthesis or its transfer to target proteins. We found GPI18, which was recently reported to encode GPI-mannosyltransferase II (GPI-MT II), as a high-copy suppressor of the temperature sensitivity of pga1ts. Both Gpi18 and Pga1 were detected in the ER by immunofluorescence, and they were coprecipitated from the Triton X-100–solubilized membrane. The gpi18ts and pga1ts mutants accumulated the same GPI synthetic intermediate at the restrictive temperature. From these results, we concluded that Pga1 is an additional essential component of the yeast GPI-MT II.

This was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E07-03-0258) on July 5, 2007.

Address correspondence to: Koji Yoda (asdfg{at}mail.ecc.u-tokyo.ac.jp).

Abbreviations used: ALP, alkaline phosphatase; CPY, carboxypeptidase Y; endoH, endoglycosidase H; ER, endoplasmic reticulum; EtN-P, phosphoethanolamine; GFP, green fluorescent protein; GlcN, glucosamine; GPI, glycosylphosphatidylinositol; GPI-MT, GPI-mannosyltransferase; Man, mannose; PI, phosphatidylinositol; SGD, Saccharomyces Genome Database.




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