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Vol. 18, Issue 9, 3512-3522, September 2007

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Serine Phosphorylation of the Integrin 4 Subunit Is Necessary for Epidermal Growth Factor Receptor–induced Hemidesmosome Disruption

Division of Cell Biology, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands

Submitted April 5, 2007; Accepted June 27, 2007
Monitoring Editor: Mark Ginsberg

Hemidesmosomes (HDs) are multiprotein adhesion complexes that promote attachment of epithelial cells to the basement membrane. The binding of 64 to plectin plays a central role in their assembly. We have defined three regions on 4 that together harbor all the serine and threonine phosphorylation sites and show that three serines (S1356, S1360, and S1364), previously implicated in HD regulation, prevent the interaction of 4 with the plectin actin-binding domain when phosphorylated. We have also established that epidermal growth factor receptor activation, which is known to function upstream of HD disassembly, results in the phosphorylation of only one or more of these three residues and the partial disassembly of HDs in keratinocytes. Additionally, we show that S1360 and S1364 of 4 are the only residues phosphorylated by PKC and PKA in cells, respectively. Taken together, our studies indicate that multiple kinases act in concert to breakdown the structural integrity of HDs in keratinocytes, which is primarily achieved through the phosphorylation of S1356, S1360, and S1364 on the 4 subunit.

The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).

Address correspondence to: Arnoud Sonnenberg (a.sonnenberg{at}nki.nl).

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