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Originally published as MBC in Press, 10.1091/mbc.E08-03-0252 on August 13, 2008

Vol. 19, Issue 10, 4454-4468, October 2008

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Swf1p, a Member of the DHHC-CRD Family of Palmitoyltransferases, Regulates the Actin Cytoskeleton and Polarized Secretion Independently of Its DHHC Motif

Shubha A. Dighe*, and Keith G. Kozminski*,{dagger}

*Department of Biology, University of Virginia, Charlottesville, VA 22904; and {dagger}Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, VA 22904

Submitted March 10, 2008; Revised July 11, 2008; Accepted August 6, 2008
Monitoring Editor: David G. Drubin

Rho and Rab family GTPases play a key role in cytoskeletal organization and vesicular trafficking, but the exact mechanisms by which these GTPases regulate polarized cell growth are incompletely understood. A previous screen for genes that interact with CDC42, which encodes a Rho GTPase, found SWF1/PSL10. Here, we show Swf1p, a member of the DHHC-CRD family of palmitoyltransferases, localizes to actin cables and cortical actin patches in Saccharomyces cerevisiae. Deletion of SWF1 results in misorganization of the actin cytoskeleton and decreased stability of actin filaments in vivo. Cdc42p localization depends upon Swf1p primarily after bud emergence. Importantly, we revealed that the actin regulating activity of Swf1p is independent of its DHHC motif. A swf1 mutant, in which alanine substituted for the cysteine required for the palmitoylation activity of DHHC-CRD proteins, displayed wild-type actin organization and Cdc42p localization. Bgl2p-marked exocytosis was found wild type in this mutant, although invertase secretion was impaired. These data indicate Swf1p has at least two distinct functions, one of which regulates actin organization and Bgl2p-marked secretion. This report is the first to link the function of a DHHC-CRD protein to Cdc42p and the regulation of the actin cytoskeleton.

This was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E08-03-0252) on August 13, 2008.

Address correspondence to: Keith G. Kozminski (kkoz{at}virginia.edu)

Abbreviations used: LatA, latrunculin A; PT, palmitoyltransferase.




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