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Originally published as MBC in Press, 10.1091/mbc.E08-05-0457 on October 8, 2008

Vol. 19, Issue 12, 5327-5337, December 2008

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SNARE Function Is Not Involved in Early Endosome Docking

Ulf Geumann*, Sina Victoria Barysch*, Peer Hoopmann*,{dagger}, Reinhard Jahn*, and Silvio O. Rizzoli*,{dagger}

*Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Göttingen 37077, Germany; and {dagger}European Neuroscience Institute, Göttingen 37077, Germany

Submitted May 6, 2008; Revised August 26, 2008; Accepted September 26, 2008
Monitoring Editor: Jean E. Gruenberg

Docking and fusion of transport vesicles constitute elementary steps in intracellular membrane traffic. While docking is thought to be initiated by Rab-effector complexes, fusion is mediated by SNARE (N-ethylmaleimide-sensitive factor [NSF] attachment receptor) proteins. However, it has been recently debated whether SNAREs also play a role in the establishment or maintenance of a stably docked state. To address this question, we have investigated the SNARE dependence of docking and fusion of early endosomes, one of the central sorting compartments in the endocytic pathway. A new, fluorescence-based in vitro assay was developed, which allowed us to investigate fusion and docking in parallel. Similar to homotypic fusion, docking of early endosomes is dependent on the presence of ATP and requires physiological temperatures. Unlike fusion, docking is insensitive to the perturbation of SNARE function by means of soluble SNARE motifs, SNARE-specific Fab fragments, or by a block of NSF activity. In contrast, as expected, docking is strongly reduced by interfering with the synthesis of phosphatidyl inositol (PI)-3 phosphate, with the function of Rab-GTPases, as well as with early endosomal autoantigen 1 (EEA1), an essential tethering factor. We conclude that docking of early endosomes is independent of SNARE function.

This was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E08-05-0457) on October 8, 2008.

Address correspondence to: Reinhard Jahn (rjahn{at}gwdg.de)




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 Proc. Natl. Acad. Sci. USAHome page
S. V. Barysch, S. Aggarwal, R. Jahn, and S. O. Rizzoli
Sorting in early endosomes reveals connections to docking- and fusion-associated factors
PNAS, June 16, 2009; 106(24): 9697 - 9702.
[Abstract] [Full Text] [PDF]


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