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Originally published as MBC in Press, 10.1091/mbc.E07-05-0504 on December 12, 2007

Vol. 19, Issue 2, 682-690, February 2008

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Biphasic Incorporation of Centromeric Histone CENP-A in Fission Yeast

Yuko Takayama, Hiroshi Sato, Shigeaki Saitoh, Yuki Ogiyama, Fumie Masuda, and Kohta Takahashi

Division of Cell Biology, Institute of Life Science, Kurume University, Fukuoka 839-0864, Japan

Submitted May 29, 2007; Revised November 12, 2007; Accepted November 27, 2007
Monitoring Editor: Kerry Bloom

CENP-A is a centromere-specific histone H3 variant that is essential for kinetochore formation. Here, we report that the fission yeast Schizosaccharomyces pombe has at least two distinct CENP-A deposition phases across the cell cycle: S and G2. The S phase deposition requires Ams2 GATA factor, which promotes histone gene activation. In {Delta}ams2, CENP-A fails to retain during S, but it reaccumulates onto centromeres via the G2 deposition pathway, which is down-regulated by Hip1, a homologue of HIRA histone chaperon. Reducing the length of G2 in {Delta}ams2 results in failure of CENP-A accumulation, leading to chromosome missegregation. N-terminal green fluorescent protein-tagging reduces the centromeric association of CENP-A, causing cell death in {Delta}ams2 but not in wild-type cells, suggesting that the N-terminal tail of CENP-A may play a pivotal role in the formation of centromeric nucleosomes at G2. These observations imply that CENP-A is normally localized to centromeres in S phase in an Ams2-dependent manner and that the G2 pathway may salvage CENP-A assembly to promote genome stability. The flexibility of CENP-A incorporation during the cell cycle may account for the plasticity of kinetochore formation when the authentic centromere is damaged.

This was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E07-05-0504) on December 12, 2007.

Address correspondence to: Kohta Takahashi (takahash{at}lsi.kurume-u.ac.jp)

Abbreviations used: ChIP, chromatin immunoprecipitation.






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