QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

Vol. 19, Issue 3, 1230-1240, March 2008

This Article Full Text Full Text (PDF) Supplemental Materials All Versions of this Article: E07-02-0130v1 19/3/1230    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Theisen, U. Articles by Steinberg, G. Search for Related Content PubMed PubMed Citation Articles by Theisen, U. Articles by Steinberg, G.

Dynamic Rearrangement of Nucleoporins during Fungal "Open" Mitosis

Ulrike Theisen^*, Anne Straube, and Gero Steinberg^*

Max-Planck-Institut für terrestrische Mikrobiologie, D-35043 Marburg, Germany

Submitted February 15, 2007; Revised November 29, 2007; Accepted December 20, 2007
Monitoring Editor: Fred Chang

Mitosis in animals starts with the disassembly of the nuclear pore complexes and the breakdown of the nuclear envelope. In contrast to many fungi, the corn smut fungus Ustilago maydis also removes the nuclear envelope. Here, we report on the dynamic behavior of the nucleoporins Nup214, Pom152, Nup133, and Nup107 in this "open" fungal mitosis. In prophase, the nuclear pore complexes disassembled and Nup214 and Pom152 dispersed in the cytoplasm and in the endoplasmic reticulum, respectively. Nup107 and Nup133 initially spread throughout the cytoplasm, but in metaphase and early anaphase occurred on the chromosomes. In anaphase, the Nup107-subcomplex redistributed to the edge of the chromosome masses, where the new envelope was reconstituted. Subsequently, Nup214 and Pom152 are recruited to the nuclear pores and protein import starts. Recruitment of nucleoporins and protein import reached a steady state in G2 phase. Formation of the nuclear envelope and assembly of nuclear pores occurred in the absence of microtubules or F-actin, but not if both were disrupted. Thus, the basic principles of nuclear pore complex dynamics seem to be conserved in organisms displaying open mitosis.

Present addresses: * School of Bioscience, Exeter University, EX4 4QD, United Kingdom;

Cytoskeletal Organization Laboratory, Marie Curie Research Institute, Oxted, RH8 0TL, United Kingdom.

Address correspondence to: Gero Steinberg (g.steinberg{at}exeter.ac.uk)

Abbreviations used: NPC, nuclear pore complex.

This article has been cited by other articles:

				K. R. Katsani, R. E. Karess, N. Dostatni, and V. Doye In Vivo Dynamics of Drosophila Nuclear Envelope Components Mol. Biol. Cell, September 1, 2008; 19(9): 3652 - 3666. [Abstract] [Full Text] [PDF]