QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

Vol. 19, Issue 3, 885-898, March 2008

This Article Full Text Full Text (PDF) Supplemental Materials All Versions of this Article: E07-10-1042v1 19/3/885    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yan, M. Articles by Subramani, S. Search for Related Content PubMed PubMed Citation Articles by Yan, M. Articles by Subramani, S.

Dysferlin Domain-containing Proteins, Pex30p and Pex31p, Localized to Two Compartments, Control the Number and Size of Oleate-induced Peroxisomes in Pichia pastoris

Mingda Yan^*, Dorian A. Rachubinski, Saurabh Joshi^*, Richard A. Rachubinski, and Suresh Subramani^*

*Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093-0322; and Department of Cell Biology, University of Alberta, Edmonton, AB T6G 2H7, Canada

Submitted October 16, 2007; Revised November 26, 2007; Accepted December 11, 2007
Monitoring Editor: Janet Shaw

Yarrowia lipolytica Pex23p and Saccharomyces cerevisiae Pex30p, Pex31p, and Pex32p comprise a family of dysferlin domain–containing peroxins. We show that the deletion of their Pichia pastoris homologues, PEX30 and PEX31, does not affect the function or division of methanol-induced peroxisomes but results in fewer and enlarged, functional, oleate-induced peroxisomes. Synthesis of Pex30p is constitutive, whereas that of Pex31p is oleate-induced but at a much lower level relative to Pex30p. Pex30p interacts with Pex31p and is required for its stability. At steady state, both Pex30p and Pex31p exhibit a dual localization to the endoplasmic reticulum (ER) and peroxisomes. However, Pex30p is localized mostly to the ER, whereas Pex31p is predominantly on peroxisomes. Consistent with ER-to-peroxisome trafficking of these proteins, Pex30p accumulates on peroxisomes upon overexpression of Pex31p. Additionally, Pex31p colocalizes with Pex30p at the ER in pex19 cells and can be chased from the ER to peroxisomes in a Pex19p-dependent manner. The dysferlin domains of Pex30p and Pex31p, which are dispensable for their interaction, stability, and subcellular localization, are essential for normal peroxisome number and size. The growth environment-specific role of these peroxins, their dual localization, and the function of their dysferlin domains provide novel insights into peroxisome morphogenesis.

Address correspondence to: Suresh Subramani (ssubramani{at}ucsd.edu)

Abbreviations used: BFP, blue fluorescent protein; BSA, bovine serum albumin; EM, electron microscopy; ER, endoplasmic reticulum; GFP, green fluorescent protein; HA, hemagglutinin; PMP, peroxisomal membrane protein; Pp, Pichia pastoris; PTS, peroxisome targeting signal; RFP, red fluorescent protein; Sc, Saccharomyces cerevisiae; YFP, yellow fluorescent protein; Yl, Yarrowia lipolytica.

This article has been cited by other articles:

				J. Chang, F. D. Mast, A. Fagarasanu, D. A. Rachubinski, G. A. Eitzen, J. B. Dacks, and R. A. Rachubinski Pex3 peroxisome biogenesis proteins function in peroxisome inheritance as class V myosin receptors J. Cell Biol., October 19, 2009; 187(2): 233 - 246. [Abstract] [Full Text] [PDF]

				T. Y. Nazarko, J.-C. Farre, and S. Subramani Peroxisome Size Provides Insights into the Function of Autophagy-related Proteins Mol. Biol. Cell, September 1, 2009; 20(17): 3828 - 3839. [Abstract] [Full Text] [PDF]

				C. Ma, U. Schumann, N. Rayapuram, and S. Subramani The Peroxisomal Matrix Import of Pex8p Requires Only PTS Receptors and Pex14p Mol. Biol. Cell, August 15, 2009; 20(16): 3680 - 3689. [Abstract] [Full Text] [PDF]