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Originally published as MBC in Press, 10.1091/mbc.E07-09-0896 on April 9, 2008

Vol. 19, Issue 6, 2673-2680, June 2008

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A Novel Group of Glutaredoxins in the cis-Golgi Critical for Oxidative Stress Resistance

Nikola Mesecke*,{dagger}, Anne Spang{ddagger}, Marcel Deponte*, and Johannes M. Herrmann{dagger}

*Institute of Physiological Chemistry, University of Munich, 81377 Munich, Germany; {ddagger}Biozentrum, University of Basel, CH-4056 Basel, Switzerland; and {dagger}Cell Biology, University of Kaiserslautern, 67663 Kaiserslautern, Germany

Submitted September 13, 2007; Revised March 25, 2008; Accepted April 2, 2008
Monitoring Editor: Jeffrey Brodsky

Glutaredoxins represent a ubiquitous family of proteins that catalyze the reduction of disulfide bonds in their substrate proteins by use of reduced glutathione. In an attempt to identify the full complement of glutaredoxins in baker's yeast, we found three so-far uncharacterized glutaredoxin-like proteins that we named Grx6, Grx7, and Grx8. Grx6 and Grx7 represent closely related monothiol glutaredoxins that are synthesized with N-terminal signal sequences. Both proteins are located in the cis-Golgi, thereby representing the first glutaredoxins found in a compartment of the secretory pathway. In contrast to formerly described monothiol glutaredoxins, Grx6 and Grx7, showed a high glutaredoxin activity in vitro. Grx6 and Grx7 overlap in their activity and deletion mutants lacking both proteins show growth defects and a strongly increased sensitivity toward oxidizing agents such as hydrogen peroxide or diamide. Our observations suggest that Grx6 and Grx7 do not play a general role in the oxidative folding of proteins in the early secretory pathway but rather counteract the oxidation of specific thiol groups in substrate proteins.

This was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E07-09-0896) on April 9, 2008.

Address correspondence to: Johannes M. Herrmann (hannes.herrmann{at}biologie.uni-kl.de)

Abbreviations used: ER, endoplasmic reticulum; GFP, green fluorescent protein; HA, hemagglutinin; HEDS, hydroxyethyl disulfide; UPR, unfolded protein response.




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Saccharomyces cerevisiae Grx6 and Grx7 Are Monothiol Glutaredoxins Associated with the Early Secretory Pathway
Eukaryot. Cell, August 1, 2008; 7(8): 1415 - 1426.
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