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Vol. 19, Issue 9, 3652-3666, September 2008

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In Vivo Dynamics of Drosophila Nuclear Envelope Components

Katerina R. Katsani^*,,, Roger E. Karess^,||,¶,#, Nathalie Dostatni^*,@, and Valérie Doye^*,

*Institut Curie, Centre de Recherche, Centre National de la Recherche Scientifique Unité Mixte de Recherche 144, ^@Centre National de la Recherche Scientifique Unité Mixte de Recherche 218, 75248 Paris Cedex 05, France; Centre National de la Recherche Scientifique, Centre de Génétique Moléculaire, Unité Propre de Recherche 2167, Gif-sur-Yvette, F-91198; ^||Université Paris-Sud, F-91405 Orsay Cedex, France; and ^¶Université Pierre et Marie Curie, F-75005, Paris, France

Submitted November 19, 2007; Revised May 19, 2008; Accepted June 11, 2008
Monitoring Editor: Susan Wente

Nuclear pore complexes (NPCs) are multisubunit protein entities embedded into the nuclear envelope (NE). Here, we examine the in vivo dynamics of the essential Drosophila nucleoporin Nup107 and several other NE-associated proteins during NE and NPCs disassembly and reassembly that take place within each mitosis. During both the rapid mitosis of syncytial embryos and the more conventional mitosis of larval neuroblasts, Nup107 is gradually released from the NE, but it remains partially confined to the nuclear (spindle) region up to late prometaphase, in contrast to nucleoporins detected by wheat germ agglutinin and lamins. We provide evidence that in all Drosophila cells, a structure derived from the NE persists throughout metaphase and early anaphase. Finally, we examined the dynamics of the spindle checkpoint proteins Mad2 and Mad1. During mitotic exit, Mad2 and Mad1 are actively imported back from the cytoplasm into the nucleus after the NE and NPCs have reformed, but they reassociate with the NE only later in G1, concomitantly with the recruitment of the basket nucleoporin Mtor (the Drosophila orthologue of vertebrate Tpr). Surprisingly, Drosophila Nup107 shows no evidence of localization to kinetochores, despite the demonstrated importance of this association in mammalian cells.

Present addresses: Department of Molecular Biology and Genetics, Democritus University of Thrace, 68100 Alexandroupolis, Greece;

^# Institut Jacques Monod, Unité Mixte de Recherche 7592, 75251 Paris, France.

Address correspondence to: Valérie Doye (vdoye{at}curie.fr)

Abbreviations used: NE, nuclear envelope; NPC, nuclear pore complex; Nup, nucleoporin; SE, spindle envelope; TLSCM, time-lapse scanning confocal microscopy; WGA, wheat germ agglutinin.

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