Molecular Biology of the Cell click for CBE Life Science Education Page

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

Originally published as MBC in Press, 10.1091/mbc.E08-04-0360 on November 12, 2008

Vol. 20, Issue 1, 556-568, January 1, 2009

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Materials
Right arrow All Versions of this Article:
E08-04-0360v1
20/1/556    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nicolas, E.
Right arrow Articles by Guichet, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nicolas, E.
Right arrow Articles by Guichet, A.

A Dual Role for Actin and Microtubule Cytoskeleton in the Transport of Golgi Units from the Nurse Cells to the Oocyte Across Ring Canals

Emmanuelle Nicolas*, Nicolas Chenouard{dagger}, Jean-Christophe Olivo-Marin{dagger}, and Antoine Guichet*

*Morphogenesis and Polarity Unit, Institut Jacques Monod, 75005 Paris, France; and {dagger}Quantitative Image Analysis Unit, Institut Pasteur, 75724 Paris, France

Submitted April 8, 2008; Revised October 27, 2008; Accepted October 30, 2008
Monitoring Editor: Vivek Malhotra

Axis specification during Drosophila embryonic development requires transfer of maternal components during oogenesis from nurse cells (NCs) into the oocyte through cytoplasmic bridges. We found that the asymmetrical distribution of Golgi, between nurse cells and the oocyte, is sustained by an active transport process. We have characterized actin basket structures that asymmetrically cap the NC side of Ring canals (RCs) connecting the oocyte. Our results suggest that these actin baskets structurally support transport mechanisms of RC transit. In addition, our tracking analysis indicates that Golgi are actively transported to the oocyte rather than diffusing. We observed that RC transit is microtubule-based and mediated at least by dynein. Finally, we show that actin networks may be involved in RC crossing through a myosin II step process, as well as in dispatching Golgi units inside the oocyte subcompartments.

This was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E08-04-0360) on November 12, 2008.

Address correspondence to: Emmanuelle Nicolas (nicolas{at}ijm.jussieu.fr) or Antoine Guichet (guichet{at}ijm.jussieu.fr)

Abbreviations used: MT, microtubule; NC, nurse cell; RC, ring canals.






Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Copyright © 2009 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.