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Originally published as MBC in Press, 10.1091/mbc.E09-03-0194 on March 18, 2009

Vol. 20, Issue 10, 2540-2548, May 15, 2009

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Polarized Growth in Budding Yeast in the Absence of a Localized Formin

Lina Gao, and Anthony Bretscher

Department of Molecular Biology and Genetics, Weill Institute for Cell and Molecular Biology, Weill Hall, Cornell University, Ithaca, NY 14853

Submitted March 9, 2009; Accepted March 11, 2009
Monitoring Editor: David G. Drubin

InCytes from MBC

Polarity is achieved partly through the localized assembly of the cytoskeleton. During growth in budding yeast, the bud cortex and neck localized formins Bni1p and Bnr1p nucleate and assemble actin cables that extend along the bud-mother axis, providing tracks for secretory vesicle delivery. Localized formins are believed to determine the location and polarity of cables, hence growth. However, yeast expressing the nonlocalized actin nucleating/assembly formin homology (FH) 1-FH2 domains of Bnr1p or Bni1p as the sole formin grow well. Although cables are significantly disorganized, analysis of directed transport of secretory vesicles is still biased toward the bud, reflecting a bias in correctly oriented cables, thereby permitting polarized growth. Myosin II, localized at the bud neck, contributes to polarized growth as a mutant unable to interact with F-actin further compromises growth in cells with an unlocalized formin but not with a localized formin. Our results show that multiple mechanisms contribute to cable orientation and polarized growth, with localized formins and myosin II being two major contributors.

This was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E09-03-0194) on March 18, 2009.

Address correspondence to: Anthony Bretscher (apb5{at}cornell.edu)


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