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Originally published as MBC in Press, 10.1091/mbc.E08-11-1115 on March 25, 2009

Vol. 20, Issue 10, 2605-2614, May 15, 2009

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Drosophila Bld10 Is a Centriolar Protein That Regulates Centriole, Basal Body, and Motile Cilium Assembly

Violaine Mottier-Pavie, and Timothy L. Megraw

Green Center for Reproductive Biology Sciences and Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9051

Submitted November 14, 2008; Revised February 23, 2009; Accepted March 17, 2009
Monitoring Editor: Yixian Zheng

Cilia and flagella play multiple essential roles in animal development and cell physiology. Defective cilium assembly or motility represents the etiological basis for a growing number of human diseases. Therefore, how cilia and flagella assemble and the processes that drive motility are essential for understanding these diseases. Here we show that Drosophila Bld10, the ortholog of Chlamydomonas reinhardtii Bld10p and human Cep135, is a ubiquitous centriolar protein that also localizes to the spermatid basal body. Mutants that lack Bld10 assemble centrioles and form functional centrosomes, but centrioles and spermatid basal bodies are short in length. bld10 mutant flies are viable but male sterile, producing immotile sperm whose axonemes are deficient in the central pair of microtubules. These results show that Drosophila Bld10 is required for centriole and axoneme assembly to confer cilium motility.

This was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E08-11-1115) on March 25, 2009.

Address correspondence to: Timothy L. Megraw (timothy.megraw{at}utsouthwestern.edu)

Abbreviations used: Asl, Asterless; Cnn, centrosomin; DSpd-2, Drosophila spindle defective 2; D-PLP, Drosophila pericentrin-like protein; GFP, green fluorescent protein; PACT, pericentrin-AKAP450 centrosomal targeting; PCM, pericentriolar material; Plk-4, Polo-like kinase 4; UNC, uncoordinated; WT, wild type; YFP, yellow fluorescent protein.




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