Molecular Biology of the Cell click for CBE Life Science Education Page

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

Originally published as MBC in Press, 10.1091/mbc.E09-02-0131 on April 8, 2009 Originally published as MBC in Press, 10.1091/mbc.E09-02-0131 on April 1, 2009

Vol. 20, Issue 11, 2650-2660, June 1, 2009

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Materials
Right arrow All Versions of this Article:
E09-02-0131v1
E09-02-0131v2
20/11/2650    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kim, J.-S.
Right arrow Articles by Bokoch, G. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kim, J.-S.
Right arrow Articles by Bokoch, G. M.

Reactive Oxygen Species Regulate a Slingshot-Cofilin Activation Pathway

Jun-Sub Kim, Timothy Y. Huang, and Gary M. Bokoch

Department of Immunology and Microbial Science and Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037

Submitted February 13, 2009; Revised March 20, 2009; Accepted March 24, 2009
Monitoring Editor: Jonathan Chernoff

Cellular stimuli generate reactive oxygen species (ROS) via the local action of NADPH oxidases (Nox) to modulate cytoskeletal organization and cell migration through unknown mechanisms. Cofilin is a major regulator of cellular actin dynamics whose activity is controlled by phosphorylation/dephosphorylation at Ser3. Here we show that Slingshot-1L (SSH-1L), a selective cofilin regulatory phosphatase, is involved in H2O2-induced cofilin dephosphorylation and activation. SSH-1L is activated by its release from a regulatory complex with 14-3-3{zeta} protein through the redox-mediated oxidation of 14-3-3{zeta} by H2O2. The ROS-dependent activation of the SSH-1L-cofilin pathway stimulates the SSH-1L–dependent formation of cofilin-actin rods in cofilin-GFP–expressing HeLa cells. Similarly, the formation of endogenous ROS stimulated by angiotensin II (AngII) also activates the SSH-1L-cofilin pathway via oxidation of 14-3-3{zeta} to increase AngII-induced membrane ruffling and cell motility. These results suggest that the formation of ROS by NADPH oxidases engages a SSH-1L-cofilin pathway to regulate cytoskeletal organization and cell migration.

This was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E09-02-0131) on April 1, 2009.

Address correspondence to: Gary M. Bokoch (bokoch{at}scripps.edu)




This article has been cited by other articles:


Home page
 J. Neurosci.Home page
I. T. Whiteman, O. L. Gervasio, K. M. Cullen, G. J. Guillemin, E. V. Jeong, P. K. Witting, S. T. Antao, L. S. Minamide, J. R. Bamburg, and C. Goldsbury
Activated Actin-Depolymerizing Factor/Cofilin Sequesters Phosphorylated Microtubule-Associated Protein during the Assembly of Alzheimer-Like Neuritic Cytoskeletal Striations
J. Neurosci., October 14, 2009; 29(41): 12994 - 13005.
[Abstract] [Full Text] [PDF]

Home page
 Sci SignalHome page
D. Gianni, B. Diaz, N. Taulet, B. Fowler, S. A. Courtneidge, and G. M. Bokoch
Novel p47phox-Related Organizers Regulate Localized NADPH Oxidase 1 (Nox1) Activity
Sci. Signal., September 15, 2009; 2(88): ra54 - ra54.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Copyright © 2009 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.