QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

Vol. 20, Issue 13, 3064-3076, July 1, 2009

This Article Full Text Full Text (PDF) Supplemental Materials All Versions of this Article: E08-12-1223v1 20/13/3064    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kugou, K. Articles by Ohta, K. Search for Related Content PubMed PubMed Citation Articles by Kugou, K. Articles by Ohta, K.

Rec8 Guides Canonical Spo11 Distribution along Yeast Meiotic Chromosomes

Kazuto Kugou^*,,, Tomoyuki Fukuda^*,, Shintaro Yamada^*, Masaru Ito^*, Hiroyuki Sasanuma^*,, Saori Mori, Yuki Katou, Takehiko Itoh^||, Kouji Matsumoto, Takehiko Shibata, Katsuhiko Shirahige, and Kunihiro Ohta^*,

*Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Tokyo 153-8902, Japan; Cellular and Molecular Biology Laboratory, RIKEN Advanced Science Institute, Wako, Saitama 351-0198, Japan; The Graduate School of Science and Engineering, Saitama University, Saitama 338-8570, Japan; Center for Biological Resources and Informatics, Division of Gene Research, and Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Kanagawa 226-8501, Japan; and ^||Laboratory of Genome Informatics, Bio-Frontier Research Center, Tokyo Institute of Technology, Yokohama, Kanagawa 226-8501, Japan

Submitted December 19, 2008; Revised April 17, 2009; Accepted May 1, 2009
Monitoring Editor: Kerry S. Bloom

Spo11-mediated DNA double-strand breaks (DSBs) that initiate meiotic recombination are temporally and spatially controlled. The meiotic cohesin Rec8 has been implicated in regulating DSB formation, but little is known about the features of their interplay. To elucidate this point, we investigated the genome-wide localization of Spo11 in budding yeast during early meiosis by chromatin immunoprecipitation using high-density tiling arrays. We found that Spo11 is dynamically localized to meiotic chromosomes. Spo11 initially accumulated around centromeres and thereafter localized to arm regions as premeiotic S phase proceeded. During this stage, a substantial proportion of Spo11 bound to Rec8 binding sites. Eventually, some of Spo11 further bound to both DSB and Rec8 sites. We also showed that such a change in a distribution of Spo11 is affected by hydroxyurea treatment. Interestingly, deletion of REC8 influences the localization of Spo11 to centromeres and in some of the intervals of the chromosomal arms. Thus, we observed a lack of DSB formation in a region-specific manner. These observations suggest that Rec8 would prearrange the distribution of Spo11 along chromosomes and will provide clues to understanding temporal and spatial regulation of DSB formation.

Address correspondence to: Kunihiro Ohta (kohta{at}bio.c.u-tokyo.ac.jp)

Abbreviations used: ChIP, chromatin immunoprecipitation; DSB, double-strand break; Gal4BD, Gal4 DNA binding domain; HU, hydroxyurea; SPM, sporulation medium.

This article has been cited by other articles:

				T. L. Callender and N. M. Hollingsworth Mek1 Suppression of Meiotic Double-Strand Break Repair Is Specific to Sister Chromatids, Chromosome Autonomous and Independent of Rec8 Cohesin Complexes Genetics, July 1, 2010; 185(3): 771 - 782. [Abstract] [Full Text] [PDF]

				L. E. Berchowitz, S. E. Hanlon, J. D. Lieb, and G. P. Copenhaver A positive but complex association between meiotic double-strand break hotspots and open chromatin in Saccharomyces cerevisiae Genome Res., December 1, 2009; 19(12): 2245 - 2257. [Abstract] [Full Text] [PDF]