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Vol. 20, Issue 15, 3561-3571, August 1, 2009

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An Intramolecular Signaling Element that Modulates Dynamin Function In Vitro and In Vivo

Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037

Submitted April 20, 2009; Revised May 27, 2009; Accepted May 29, 2009
Monitoring Editor: Janet M. Shaw

Dynamin exhibits a high basal rate of GTP hydrolysis that is enhanced by self-assembly on a lipid template. Dynamin's GTPase effector domain (GED) is required for this stimulation, though its mechanism of action is poorly understood. Recent structural work has suggested that GED may physically dock with the GTPase domain to exert its stimulatory effects. To examine how these interactions activate dynamin, we engineered a minimal GTPase-GED fusion protein (GG) that reconstitutes dynamin's basal GTPase activity and utilized it to define the structural framework that mediates GED's association with the GTPase domain. Chemical cross-linking of GG and mutagenesis of full-length dynamin establishes that the GTPase-GED interface is comprised of the N- and C-terminal helices of the GTPase domain and the C-terminus of GED. We further show that this interface is essential for structural stability in full-length dynamin. Finally, we identify mutations in this interface that disrupt assembly-stimulated GTP hydrolysis and dynamin-catalyzed membrane fission in vitro and impair the late stages of clathrin-mediated endocytosis in vivo. These data suggest that the components of the GTPase-GED interface act as an intramolecular signaling module, which we term the bundle signaling element, that can modulate dynamin function in vitro and in vivo.

* Present address: Laboratory of Molecular Biology, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892.

Address correspondence to: Sandra L. Schmid (slschmid{at}scripps.edu)

Abbreviations used: Dyn 2, dynamin 2; BSS-Tfn, biotinylated-transferrin; GED, GTPase effector domain; GG, minimal GTPase-GED fusion; MTS, methanthiosulfonate; PIP₂, L--phosphatidylinositol-4,5-bisphosphate.

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