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Originally published as MBC in Press, 10.1091/mbc.E09-04-0330 on July 15, 2009

Vol. 20, Issue 17, 3818-3827, September 1, 2009

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Pericentromeric Sister Chromatid Cohesion Promotes Kinetochore Biorientation

Tessie M. Ng*,{dagger}, William G. Waples{ddagger}, Brigitte D. Lavoie{ddagger}, and Sue Biggins*

*Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109; {dagger}Molecular and Cellular Biology Program, University of Washington and Fred Hutchinson Cancer Research Center, Seattle, WA 98195; and {ddagger}Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada M5S 1A8

Submitted April 27, 2009; Revised June 25, 2009; Accepted July 2, 2009
Monitoring Editor: Kerry S. Bloom

InCytes from MBC

Accurate chromosome segregation depends on sister kinetochores making bioriented attachments to microtubules from opposite poles. An essential regulator of biorientation is the Ipl1/Aurora B protein kinase that destabilizes improper microtubule–kinetochore attachments. To identify additional biorientation pathways, we performed a systematic genetic analysis between the ipl1-321 allele and all nonessential budding yeast genes. One of the mutants, mcm21{Delta}, precociously separates pericentromeres and this is associated with a defect in the binding of the Scc2 cohesin-loading factor at the centromere. Strikingly, Mcm21 becomes essential for biorientation when Ipl1 function is reduced, and this appears to be related to its role in pericentromeric cohesion. When pericentromeres are artificially tethered, Mcm21 is no longer needed for biorientation despite decreased Ipl1 activity. Taken together, these data reveal a specific role for pericentromeric linkage in ensuring kinetochore biorientation.

This was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E09-04-0330) on July 15, 2009.

Address correspondence to: Sue Biggins (sbiggins{at}fhcrc.org)

Abbreviations used: MT, microtubule; NC-Mcd1, noncleavable Mcd1; SGA, systematic genetic analysis.




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Analysis of Ipl1-Mediated Phosphorylation of the Ndc80 Kinetochore Protein in Saccharomyces cerevisiae
Genetics, December 1, 2009; 183(4): 1591 - 1595.
[Abstract] [Full Text] [PDF]


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