QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

Vol. 20, Issue 17, 3851-3864, September 1, 2009

This Article Full Text Full Text (PDF) Supplemental Materials All Versions of this Article: E09-04-0347v1 20/17/3851    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Li, L. Articles by Breeden, L. L. Search for Related Content PubMed PubMed Citation Articles by Li, L. Articles by Breeden, L. L.

Budding Yeast SSD1-V Regulates Transcript Levels of Many Longevity Genes and Extends Chronological Life Span in Purified Quiescent Cells

Lihong Li^*, Yong Lu^,, Li-Xuan Qin, Ziv Bar-Joseph, Margaret Werner-Washburne^||, and Linda L. Breeden^*

*Fred Hutchinson Cancer Research Center, Basic Sciences Division, Seattle, WA 98109; Carnegie Mellon University, School of Computer Science and Lane Center for Computational Biology, Pittsburgh, PA 15213; Memorial Sloan-Kettering Cancer Center, Epidemiology and Biostatistics, New York, NY 10021; and ^||University of New Mexico, Department of Biology, Albuquerque, NM 87131

Submitted April 30, 2009; Accepted June 24, 2009
Monitoring Editor: Charles Boone

Ssd1 is an RNA-binding protein that affects literally hundreds of different processes and is polymorphic in both wild and lab yeast strains. We have used transcript microarrays to compare mRNA levels in an isogenic pair of mutant (ssd1-d) and wild-type (SSD1-V) cells across the cell cycle. We find that 15% of transcripts are differentially expressed, but there is no correlation with those mRNAs bound by Ssd1. About 20% of cell cycle regulated transcripts are affected, and most show sharper amplitudes of oscillation in SSD1-V cells. Many transcripts whose gene products influence longevity are also affected, the largest class of which is involved in translation. Ribosomal protein mRNAs are globally down-regulated by SSD1-V. SSD1-V has been shown to increase replicative life span¤ and we show that SSD1-V also dramatically increases chronological life span (CLS). Using a new assay of CLS in pure populations of quiescent prototrophs, we find that the CLS for SSD1-V cells is twice that of ssd1-d cells.

Present address: Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 250 Longwood Ave, Boston, MA 02115.

Address correspondence to: Linda L. Breeden (lbreeden{at}fhcrc.org)