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Originally published as MBC in Press, 10.1091/mbc.E09-05-0359 on August 5, 2009

Vol. 20, Issue 19, 4131-4139, October 1, 2009

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Function and Assembly of DNA Looping, Clustering, and Microtubule Attachment Complexes within a Eukaryotic Kinetochore

Marybeth Anderson, Julian Haase, Elaine Yeh, and Kerry Bloom

Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-3280

Submitted May 1, 2009; Revised July 21, 2009; Accepted July 23, 2009
Monitoring Editor: David G. Drubin

InCytes from MBC

The kinetochore is a complex protein–DNA assembly that provides the mechanical linkage between microtubules and the centromere DNA of each chromosome. Centromere DNA in all eukaryotes is wrapped around a unique nucleosome that contains the histone H3 variant CENP-A (Cse4p in Saccharomyces cerevisiae). Here, we report that the inner kinetochore complex (CBF3) is required for pericentric DNA looping at the Cse4p-containing nucleosome. DNA within the pericentric loop occupies a spatially confined area that is radially displaced from the interpolar central spindle. Microtubule-binding kinetochore complexes are not involved in pericentric DNA looping but are required for the geometric organization of DNA loops around the spindle microtubules in metaphase. Thus, the mitotic segregation apparatus is a composite structure composed of kinetochore and interpolar microtubules, the kinetochore, and organized pericentric DNA loops. The linkage of microtubule-binding to centromere DNA-looping complexes positions the pericentric chromatin loops and stabilizes the dynamic properties of individual kinetochore complexes in mitosis.

This was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E09-05-0359) on August 5, 2009.

Address correspondence to: Kerry Bloom (kerry_bloom{at}unc.edu).






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