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Originally published as MBC in Press, 10.1091/mbc.E09-04-0309 on September 30, 2009

Vol. 20, Issue 22, 4804-4815, November 15, 2009

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Telomeric DNA Mediates De Novo PML Body Formation

Anneke K. Brouwer*, Joost Schimmel*, Joop C.A.G. Wiegant*, Alfred C.O. Vertegaal*, Hans J. Tanke*, and Roeland W. Dirks*

*Department of Molecular Cell Biology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands

Submitted April 16, 2009; Revised August 12, 2009; Accepted September 21, 2009
Monitoring Editor: A. Gregory Matera

The cell nucleus harbors a variety of different bodies that vary in number, composition, and size. Although these bodies coordinate important nuclear processes, little is known about how they are formed. Among the most intensively studied bodies in recent years is the PML body. These bodies have been implicated in gene regulation and other cellular processes and are disrupted in cells from patients suffering from acute promyelocytic leukemia. Using live cell imaging microscopy and immunofluorescence, we show in several cell types that PML bodies are formed at telomeric DNA during interphase. Recent studies revealed that both SUMO modification sites and SUMO interaction motifs in the promyelocytic leukemia (PML) protein are required for PML body formation. We show that SMC5, a component of the SUMO ligase MMS21-containing SMC5/6 complex, localizes temporarily at telomeric DNA during PML body formation, suggesting a possible role for SUMO in the formation of PML bodies at telomeric DNA. Our data identify a novel role of telomeric DNA during PML body formation.

This was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E09-04-0309) on September 30, 2009.

Address correspondence to: Roeland W. Dirks, Department of Molecular Cell Biology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands (r.w.dirks{at}lumc.nl)

Abbreviations used: FISH, fluorescence in situ hybridization; MEF, mouse embryonic fibroblast; MMS, methylmethane sulfonate; PML, promyelocytic leukemia; PNA, peptide nucleic acid; SUMO, small ubiquitin-like modifier; TRF, telomeric repeat binding factor.






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