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Originally published as MBoC in Press, 10.1091/mbc.E08-09-0917 on January 21, 2009

Vol. 20, Issue 6, 1629-1638, March 15, 2009

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RACK-1 Directs Dynactin-dependent RAB-11 Endosomal Recycling during Mitosis in Caenorhabditis elegans

Erkang Ai, Daniel S. Poole, and Ahna R. Skop

Laboratory of Genetics, University of Wisconsin–Madison, Madison, WI 53706

Submitted September 9, 2008; Revised December 14, 2008; Accepted January 8, 2009
Monitoring Editor: Yu-Li Wang

Membrane trafficking pathways are necessary for the addition and removal of membrane during cytokinesis. In animal cells, recycling endosomes act as a major source of the additional membranes during furrow progression and abscission. However, the mechanisms and factors that regulate recycling endosomes during the cell cycle remain poorly understood. Here, we show that the Caenorhabditis elegans Receptor of Activated C Kinase 1 (RACK-1) is required for cytokinesis, germline membrane organization, and the recruitment of RAB-11–labeled recycling endosomes to the pericentrosomal region and spindle. RACK-1 is also required for proper chromosome separation and astral microtubule length. RACK-1 localizes to the centrosomes, kinetochores, the midbody, and nuclear envelopes during the cell cycle. We found that RACK-1 directly binds to DNC-2, the C. elegans p50/dynamitin subunit of the dynactin complex. Last, RACK-1 may facilitate the sequestration of recycling endosomes by targeting DNC-2 to centrosomes and the spindle. Our findings suggest a mechanism by which RACK-1 directs the dynactin-dependent redistribution of recycling endosomes during the cell cycle, thus ensuring proper membrane trafficking events during cytokinesis.

This was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E08-09-0917) on January 21, 2009.

Address correspondence to: Ahna R. Skop (skop{at}wisc.edu)

Abbreviations used: RE, recycling endosome; DIC, differential interference contrast; WT, wild type.




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