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Originally published as MBC in Press, 10.1091/mbc.E08-12-1169 on February 18, 2009

Vol. 20, Issue 8, 2311-2326, April 15, 2009

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Regulation of Distinct Septin Rings in a Single Cell by Elm1p and Gin4p Kinases

Bradley S. DeMay, Rebecca A. Meseroll, Patricia Occhipinti, and Amy S. Gladfelter

Department of Biological Sciences, Dartmouth College, Hanover, NH 03755

Submitted December 4, 2008; Revised January 21, 2009; Accepted February 10, 2009
Monitoring Editor: Patrick J. Brennwald

Septins are conserved, GTP-binding proteins that assemble into higher order structures, including filaments and rings with varied cellular functions. Using four-dimensional quantitative fluorescence microscopy of Ashbya gossypii fungal cells, we show that septins can assemble into morphologically distinct classes of rings that vary in dimensions, intensities, and positions within a single cell. Notably, these different classes coexist and persist for extended times, similar in appearance and behavior to septins in mammalian neurons and cultured cells. We demonstrate that new septin proteins can add through time to assembled rings, indicating that septins may continue to polymerize during ring maturation. Different classes of rings do not arise from the presence or absence of specific septin subunits and ring maintenance does not require the actin and microtubule cytoskeletons. Instead, morphological and behavioral differences in the rings require the Elm1p and Gin4p kinases. This work demonstrates that distinct higher order septin structures form within one cell because of the action of specific kinases.

This was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E08-12-1169) on February 18, 2009.

Address correspondence to: Amy S. Gladfelter (amy.gladfelter{at}dartmouth.edu)




This article has been cited by other articles:


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R. Lindsey, S. Cowden, Y. Hernandez-Rodriguez, and M. Momany
Septins AspA and AspC Are Important for Normal Development and Limit the Emergence of New Growth Foci in the Multicellular Fungus Aspergillus nidulans
Eukaryot. Cell, January 1, 2010; 9(1): 155 - 163.
[Abstract] [Full Text] [PDF]


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