![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
JS Mymryk, RW Lee and ST Bayley
Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada.
We have used deletion mutants to define the regions in Ad5 E1A proteins necessary to suppress differentiation of mouse BC3H1 myoblasts. We examined the differentiation of cells infected at a low multiplicity with viruses containing the E1A deletions and constructed so as to produce only the smaller of the two major E1A proteins. Only four of the mutant viruses containing deletions within the N-terminal 69 residues failed to suppress differentiation as judged by changes in morphology and in levels of muscle-specific alpha-actin mRNA and creatine kinase activity. The results were confirmed by analyses of lines of cells stably transfected with representative E1A mutants. The mouse cellular proteins to which mutant E1A proteins bound were identified by immunoprecipitating E1A proteins specifically from infected BC3H1 cells and by analyzing the precipitates on denaturing gels. Bands of proteins of 300, 130, 107, 105 (the retinoblastoma product), and 60 kDa (cyclin A) were distinguished. Failure to suppress differentiation correlated with loss of binding to the 300-kDa protein but not to any of the others. The regions of E1A defined in this way have been shown to be required for several other activities, including enhancer repression and transformation. One function of the 300-kDa protein appears to be to facilitate the action of transcriptional enhancers of differentiation-specific genes.
This article has been cited by other articles:
|
|
 |
|
  H. Yang, W. Wei, M. Menconi, and P.-O. Hasselgren Dexamethasone-induced protein degradation in cultured myotubes is p300/HAT dependent Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2007; 292(1): R337 - R334. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Ma, J. K. L. Chan, G. Zhu, and Z. Wu Myocyte Enhancer Factor 2 Acetylation by p300 Enhances Its DNA Binding Activity, Transcriptional Activity, and Myogenic Differentiation Mol. Cell. Biol., May 1, 2005; 25(9): 3575 - 3582. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Zhang, A. S. Turnell, C. Gorbea, J. S. Mymryk, P. H. Gallimore, and R. J. A. Grand The Targeting of the Proteasomal Regulatory Subunit S2 by Adenovirus E1A Causes Inhibition of Proteasomal Activity and Increased p53 Expression J. Biol. Chem., June 11, 2004; 279(24): 25122 - 25133. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. H. Goodman and S. Smolik CBP/p300 in cell growth, transformation, and development Genes & Dev., July 1, 2000; 14(13): 1553 - 1577. [Full Text]
|
|
|
|
 |
|
 A. L. Adamson and S. Kenney The Epstein-Barr Virus BZLF1 Protein Interacts Physically and Functionally with the Histone Acetylase CREB-Binding Protein J. Virol., August 1, 1999; 73(8): 6551 - 6558. [Abstract] [Full Text]
|
|
|
|
|
|
C. Cenciarelli, F. De Santa, P. L. Puri, E. Mattei, L. Ricci, F. Bucci, A. Felsani, and M. Caruso Critical Role Played by Cyclin D3 in the MyoD-Mediated Arrest of Cell Cycle during Myoblast Differentiation Mol. Cell. Biol., July 1, 1999; 19(7): 5203 - 5217. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. O'Connor, H. Zimmermann, S. Nielsen, H.-U. Bernard, and T. Kouzarides Characterization of an E1A-CBP Interaction Defines a Novel Transcriptional Adapter Motif (TRAM) in CBP/p300 J. Virol., May 1, 1999; 73(5): 3574 - 3581. [Abstract] [Full Text]
|
|
|
|
|
|
Y. Tsuji, E. Moran, S. V. Torti, and F. M. Torti Transcriptional Regulation of the Mouse Ferritin H Gene. INVOLVEMENT OF p300/CBP ADAPTOR PROTEINS IN FER-1 ENHANCER ACTIVITY J. Biol. Chem., March 12, 1999; 274(11): 7501 - 7507. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. B. Dallas, I. W. Cheney, D.-W. Liao, V. Bowrin, W. Byam, S. Pacchione, R. Kobayashi, P. Yaciuk, and E. Moran p300/CREB Binding Protein-Related Protein p270 Is a Component of Mammalian SWI/SNF Complexes Mol. Cell. Biol., June 1, 1998; 18(6): 3596 - 3603. [Abstract] [Full Text]
|
|
|
|
 |
|
 G. M. Fimia, V. Gottifredi, B. Bellei, M. R. Ricciardi, A. Tafuri, P. Amati, and R. Maione The Activity of Differentiation Factors Induces Apoptosis in Polyomavirus Large T-Expressing Myoblasts Mol. Biol. Cell, June 1, 1998; 9(6): 1449 - 1463. [Abstract] [Full Text]
|
|
|
|
|
|
Y. Shi and C. Mello A CBP/p300 homolog specifies multiple differentiation pathways in Caenorhabditis elegans Genes & Dev., April 1, 1998; 12(7): 943 - 955. [Abstract] [Full Text]
|
|
|
|
|
|
N. H. Bishopric, G.-Q. Zeng, B. Sato, and K. A. Webster Adenovirus E1A Inhibits Cardiac Myocyte-specific Gene Expression through Its Amino Terminus J. Biol. Chem., August 15, 1997; 272(33): 20584 - 20594. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R Eckner, T P Yao, E Oldread, and D M Livingston Interaction and functional collaboration of p300/CBP and bHLH proteins in muscle and B-cell differentiation. Genes & Dev., October 1, 1996; 10(19): 2478 - 2490. [Abstract] [PDF]
|
|
|
|
|
|
W. Yuan, G. Condorelli, M. Caruso, A. Felsani, and A. Giordano Human p300 Protein Is a Coactivator for the Transcription Factor MyoD J. Biol. Chem., April 12, 1996; 271(15): 9009 - 9013. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. H. See, D. Calvo, Y. Shi, H. Kawa, M. P.-S. Luke, Z. Yuan, and Y. Shi Stimulation of p300-mediated Transcription by the Kinase MEKK1 J. Biol. Chem., May 4, 2001; 276(19): 16310 - 16317. [Abstract] [Full Text] [PDF]
|
|
