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I Fitch, C Dahmann, U Surana, A Amon, K Nasmyth, L Goetsch, B Byers and B Futcher
Cold Spring Harbor Laboratory, New York 11724.
The previously described CLB1 and CLB2 genes encode a closely related pair of B-type cyclins. Here we present the sequences of another related pair of B-type cyclin genes, which we term CLB3 and CLB4. Although CLB1 and CLB2 mRNAs rise in abundance at the time of nuclear division, CLB3 and CLB4 are turned on earlier, rising early in S phase and declining near the end of nuclear division. When all possible single and multiple deletion mutants were constructed, some multiple mutations were lethal, whereas all single mutants were viable. All lethal combinations included the clb2 deletion, whereas the clb1 clb3 clb4 triple mutant was viable, suggesting a key role for CLB2. The inviable multiple clb mutants appeared to have a defect in mitosis. Conditional clb mutants arrested as large budded cells with a G2 DNA content but without any mitotic spindle. Electron microscopy showed that the spindle pole bodies had duplicated but not separated, and no spindle had formed. This suggests that the Clb/Cdc28 kinase may have a relatively direct role in spindle formation. The two groups of Clbs may have distinct roles in spindle formation and elongation.
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|
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|
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|
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|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
 |
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
C. S. Detweiler and J. J. Li Ectopic induction of Clb2 in early G1 phase is sufficient to block prereplicative complex formation in Saccharomyces cerevisiae PNAS, March 3, 1998; 95(5): 2384 - 2389. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Altman and D. Kellogg Control of Mitotic Events by Nap1 and the Gin4 Kinase J. Cell Biol., July 14, 1997; 138(1): 119 - 130. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-L. Juang, J. Huang, J.-M. Peters, M. E. McLaughlin, C.-Y. Tai, and D. Pellman APC-Mediated Proteolysis of Ase1 and the Morphogenesis of the Mitotic Spindle Science, February 28, 1997; 275(5304): 1311 - 1314. [Abstract] [Full Text]
|
|
|
|
|
|
R. S. Cuevo, S. Garrett, and J. M. Horowitz Detection and Functional Characterization of p180, a Novel Cell Cycle Regulated Yeast Transcription Factor That Binds Retinoblastoma Control Elements J. Biol. Chem., February 7, 1997; 272(6): 3813 - 3822. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S Irniger and K Nasmyth The anaphase-promoting complex is required in G1 arrested yeast cells to inhibit B-type cyclin accumulation and to prevent uncontrolled entry into S-phase J. Cell Sci., January 7, 1997; 110(13): 1523 - 1531. [Abstract] [PDF]
|
|
|
|
|
|
J F Diffley Once and only once upon a time: specifying and regulating origins of DNA replication in eukaryotic cells. Genes & Dev., November 15, 1996; 10(22): 2819 - 2830. [PDF]
|
|
|
|
|
|
S Piatti, T Bohm, J H Cocker, J F Diffley, and K Nasmyth Activation of S-phase-promoting CDKs in late G1 defines a "point of no return" after which Cdc6 synthesis cannot promote DNA replication in yeast. Genes & Dev., June 15, 1996; 10(12): 1516 - 1531. [Abstract] [PDF]
|
|
|
|
 |
|
 D. Ruden and H Jackle Mitotic delay dependent survival identifies components of cell cycle control in the Drosophila blastoderm Development, January 1, 1995; 121(1): 63 - 73. [Abstract] [PDF]
|
|
|
|
|
|
M Hatakeyama, J A Brill, G R Fink, and R A Weinberg Collaboration of G1 cyclins in the functional inactivation of the retinoblastoma protein. Genes & Dev., August 1, 1994; 8(15): 1759 - 1771. [Abstract] [PDF]
|
|
|
|
|
|
S. Forsburg and P Nurse Analysis of the Schizosaccharomyces pombe cyclin puc1: evidence for a role in cell cycle exit J. Cell Sci., January 3, 1994; 107(3): 601 - 613. [Abstract] [PDF]
|
|
|
|
|
|
Y Tang and S I Reed The Cdk-associated protein Cks1 functions both in G1 and G2 in Saccharomyces cerevisiae. Genes & Dev., May 1, 1993; 7(5): 822 - 832. [Abstract] [PDF]
|
|
|
|
|
|
D. Chapman and D. Wolgemuth Isolation of the murine cyclin B2 cDNA and characterization of the lineage and temporal specificity of expression of the B1 and B2 cyclins during oogenesis, spermatogenesis and early embryogenesis Development, January 5, 1993; 118(1): 229 - 240. [Abstract] [PDF]
|
|
