![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
MA Bossie, C DeHoratius, G Barcelo and P Silver
Department of Molecular Biology, Princeton University, New Jersey 08544.
We have isolated mutants of the yeast Saccharomyces cerevisiae that are defective in localization of nuclear proteins. Chimeric proteins containing the nuclear localization sequence from SV40 large T-antigen fused to the N-terminus of the mitochondrial F1 beta-ATPase are localized to the nucleus. Npl (nuclear protein localization) mutants were isolated by their ability to grow on glycerol as a consequence of no longer exclusively targeting SV40-F1 beta-ATPase to the nucleus. All mutants with defects in localization of nucleolar proteins and histones are temperature sensitive for growth at 36 degrees C. Seven alleles of NPL3 and single alleles of several additional genes were isolated. NPL3 mutants were studied in detail. NPL3 encodes a nuclear protein with an RNA recognition motif and similarities to a family of proteins involved in RNA metabolism. Our genetic analysis indicates that NPL3 is essential for normal cell growth; cells lacking NPL3 are temperature sensitive for growth but do not exhibit a defect in localization of nuclear proteins. Taken together, these results indicate that the mutant forms of Npl3 protein isolated by this procedure are interfering with nuclear protein uptake in a general manner.
This article has been cited by other articles:
|
|
 |
|
  M. K. Lund, T. L. Kress, and C. Guthrie Autoregulation of Npl3, a Yeast SR Protein, Requires a Novel Downstream Region and Serine Phosphorylation Mol. Cell. Biol., June 1, 2008; 28(11): 3873 - 3881. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Balzer and M. F. Henry Snu56p Is Required for Mer1p-Activated Meiotic Splicing Mol. Cell. Biol., April 15, 2008; 28(8): 2497 - 2508. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. E. McBride, C. Zurita-Lopez, A. Regis, E. Blum, A. Conboy, S. Elf, and S. Clarke Protein Arginine Methylation in Candida albicans: Role in Nuclear Transport Eukaryot. Cell, July 1, 2007; 6(7): 1119 - 1129. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Wilson, H. Erdjument-Bromage, P. Tempst, and B. R. Cairns The RSC Chromatin Remodeling Complex Bears an Essential Fungal-Specific Protein Module With Broad Functional Roles Genetics, February 1, 2006; 172(2): 795 - 809. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. E. McBride, J. T. Cook, E. A. Stemmler, K. L. Rutledge, K. A. McGrath, and J. A. Rubens Arginine Methylation of Yeast mRNA-binding Protein Npl3 Directly Affects Its Function, Nuclear Export, and Intranuclear Protein Interactions J. Biol. Chem., September 2, 2005; 280(35): 30888 - 30898. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Savchenko, N. Krogan, J. R. Cort, E. Evdokimova, J. M. Lew, A. A. Yee, L. Sanchez-Pulido, M. A. Andrade, A. Bochkarev, J. D. Watson, et al. The Shwachman-Bodian-Diamond Syndrome Protein Family Is Involved in RNA Metabolism J. Biol. Chem., May 13, 2005; 280(19): 19213 - 19220. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Xu and M. F. Henry Nuclear Export of hnRNP Hrp1p and Nuclear Export of hnRNP Npl3p Are Linked and Influenced by the Methylation State of Npl3p Mol. Cell. Biol., December 15, 2004; 24(24): 10742 - 10756. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Hayano, M. Yanagida, Y. Yamauchi, T. Shinkawa, T. Isobe, and N. Takahashi Proteomic Analysis of Human Nop56p-associated Pre-ribosomal Ribonucleoprotein Complexes: POSSIBLE LINK BETWEEN Nop56p AND THE NUCLEOLAR PROTEIN TREACLE RESPONSIBLE FOR TREACHER COLLINS SYNDROME J. Biol. Chem., September 5, 2003; 278(36): 34309 - 34319. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. F. Henry, D. Mandel, V. Routson, and P. A. Henry The Yeast hnRNP-like Protein Hrp1/Nab4 Accumulates in the Cytoplasm after Hyperosmotic Stress: A Novel Fps1-dependent Response Mol. Biol. Cell, September 1, 2003; 14(9): 3929 - 3941. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. XU, P. A. HENRY, A. SETYA, and M. F. HENRY In vivo analysis of nucleolar proteins modified by the yeast arginine methyltransferase Hmt1/Rmt1p RNA, June 1, 2003; 9(6): 746 - 759. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Misra, C. Qi, S. Yu, S. H. Shah, W.-Q. Cao, M. S. Rao, B. Thimmapaya, Y. Zhu, and J. K. Reddy Interaction of PIMT with Transcriptional Coactivators CBP, p300, and PBP Differential Role in Transcriptional Regulation J. Biol. Chem., May 24, 2002; 277(22): 20011 - 20019. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Green, K. A. Marfatia, E. B. Crafton, X. Zhang, X. Cheng, and A. H. Corbett Nab2p Is Required for Poly(A) RNA Export in Saccharomyces cerevisiae and Is Regulated by Arginine Methylation via Hmt1p J. Biol. Chem., March 1, 2002; 277(10): 7752 - 7760. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Hitchcock, H. Krebber, S. Frietze, A. Lin, M. Latterich, and P. A. Silver The Conserved Npl4 Protein Complex Mediates Proteasome-dependent Membrane-bound Transcription Factor Activation Mol. Biol. Cell, October 1, 2001; 12(10): 3226 - 3241. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. P. Lei, H. Krebber, and P. A. Silver Messenger RNAs are recruited for nuclear export during transcription Genes & Dev., July 15, 2001; 15(14): 1771 - 1782. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Ni and M. Snyder A Genomic Study of the Bipolar Bud Site Selection Pattern in Saccharomyces cerevisiae Mol. Biol. Cell, July 1, 2001; 12(7): 2147 - 2170. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. McBride, V. H. Weiss, H. K. Kim, J. M. Hogle, and P. A. Silver Analysis of the Yeast Arginine Methyltransferase Hmt1p/Rmt1p and Its in Vivo Function. COFACTOR BINDING AND SUBSTRATE INTERACTIONS J. Biol. Chem., February 4, 2000; 275(5): 3128 - 3136. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Kressler, P. Linder, and J. de la Cruz Protein trans-Acting Factors Involved in Ribosome Biogenesis in Saccharomyces cerevisiae Mol. Cell. Biol., December 1, 1999; 19(12): 7897 - 7912. [Full Text] [PDF]
|
|
|
|
|
|
T. I. Moy and P. A. Silver Nuclear export of the small ribosomal subunit requires the Ran-GTPase cycle and certain nucleoporins Genes & Dev., August 15, 1999; 13(16): 2118 - 2133. [Abstract] [Full Text]
|
|
|
|
|
|
H. Krebber, T. Taura, M. S. Lee, and P. A. Silver Uncoupling of the hnRNP Npl3p from mRNAs during the stress-induced block in mRNA export Genes & Dev., August 1, 1999; 13(15): 1994 - 2004. [Abstract] [Full Text]
|
|
|
|
 |
|
 N. Kataoka, J. L. Bachorik, and G. Dreyfuss Transportin-SR, a Nuclear Import Receptor for SR Proteins J. Cell Biol., June 14, 1999; 145(6): 1145 - 1152. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Zhao, L. Hyman, and C. Moore Formation of mRNA 3' Ends in Eukaryotes: Mechanism, Regulation, and Interrelationships with Other Steps in mRNA Synthesis Microbiol. Mol. Biol. Rev., June 1, 1999; 63(2): 405 - 445. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Solsbacher, P. Maurer, F. R. Bischoff, and G. Schlenstedt Cse1p Is Involved in Export of Yeast Importin alpha from the Nucleus Mol. Cell. Biol., November 1, 1998; 18(11): 6805 - 6815. [Abstract] [Full Text]
|
|
|
|
|
|
F. Stutz and M. Rosbash Nuclear RNA export Genes & Dev., November 1, 1998; 12(21): 3303 - 3319. [Full Text]
|
|
|
|
|
|
E. C. Shen, M. F. Henry, V. H. Weiss, S. R. Valentini, P. A. Silver, and M. S. Lee Arginine methylation facilitates the nuclear export of hnRNP proteins Genes & Dev., March 1, 1998; 12(5): 679 - 691. [Abstract] [Full Text]
|
|
|
|
|
|
L. F. Pemberton, J. S. Rosenblum, and G. Blobel A Distinct and Parallel Pathway for the Nuclear Import of an mRNA-binding Protein J. Cell Biol., December 29, 1997; 139(7): 1645 - 1653. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Taura, G. Schlenstedt, and P. A. Silver Yrb2p Is a Nuclear Protein That Interacts with Prp20p, a Yeast Rcc1 Homologue J. Biol. Chem., December 12, 1997; 272(50): 31877 - 31884. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Kessler, M. F. Henry, E. Shen, J. Zhao, S. Gross, P. A. Silver, and C. L. Moore Hrp1, a sequence-specific RNA-binding protein that shuttles between the nucleus and the cytoplasm, is required for mRNA 3'-end formation in yeast Genes & Dev., October 1, 1997; 11(19): 2545 - 2556. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Seedorf and P. A. Silver Importin/karyopherin protein family members required for mRNA export from the nucleus PNAS, August 5, 1997; 94(16): 8590 - 8595. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Izaurralde, A. Jarmolowski, C. Beisel, I. W. Mattaj, G. Dreyfuss, and U. Fischer A Role for the M9 Transport Signal of hnRNP A1 in mRNA Nuclear Export J. Cell Biol., April 7, 1997; 137(1): 27 - 35. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. H. Corbett and P. A. Silver The NTF2 Gene Encodes an Essential, Highly Conserved Protein That Functions in Nuclear Transport in Vivo J. Biol. Chem., August 2, 1996; 271(31): 18477 - 18484. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C Saavedra, K S Tung, D C Amberg, A K Hopper, and C N Cole Regulation of mRNA export in response to stress in Saccharomyces cerevisiae. Genes & Dev., July 1, 1996; 10(13): 1608 - 1620. [Abstract] [PDF]
|
|
|
|
|
|
J. D. Gary, W.-J. Lin, M. C. Yang, H. R. Herschman, and S. Clarke The Predominant Protein-arginine Methyltransferase from Saccharomyces cerevisiae J. Biol. Chem., May 24, 1996; 271(21): 12585 - 12594. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M S Lee, M Henry, and P A Silver A protein that shuttles between the nucleus and the cytoplasm is an important mediator of RNA export. Genes & Dev., May 15, 1996; 10(10): 1233 - 1246. [Abstract] [PDF]
|
|
|
|
 |
|
 D. Singleton, S Chen, M Hitomi, C Kumagai, and A. Tartakoff A yeast protein that bidirectionally affects nucleocytoplasmic transport J. Cell Sci., January 1, 1995; 108(1): 265 - 272. [Abstract] [PDF]
|
|
|
|
|
|
U Stochaj, M. Bossie, K van Zee, A. Whalen, and P. Silver Analysis of conserved binding proteins for nuclear localization sequences J. Cell Sci., January 1, 1993; 104(1): 89 - 95. [Abstract] [PDF]
|
|
|
|
|
|
E. C. Shen, T. Stage-Zimmermann, P. Chui, and P. A. Silver 7The Yeast mRNA-binding Protein Npl3p Interacts with the Cap-binding Complex J. Biol. Chem., July 28, 2000; 275(31): 23718 - 23724. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Inoue, T. Mizuno, K. Wada, and M. Hagiwara Novel RING Finger Proteins, Air1p and Air2p, Interact with Hmt1p and Inhibit the Arginine Methylation of Npl3p J. Biol. Chem., October 13, 2000; 275(42): 32793 - 32799. [Abstract] [Full Text] [PDF]
|
|
