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T Fujimori and M Takeichi
Department of Biophysics, Faculty of Science, Kyoto University, Japan.
Cadherins, a family of transmembrane cell-cell adhesion receptors, require interactions with the cytoskeleton for normal function. To assess the mechanisms of these interactions, we studied the effect of exogenous expression of a mutant N-cadherin, cN390 delta; on epithelial cell-cell adhesion. The intracellular domain of cN390 delta was intact but its extracellular domain was largely deleted so that this molecule was not functional for cell adhesion. cDNA of cN390 delta was attached to the metallothionein promoter, and introduced into the keratinocyte line PAM212 expressing endogenous E- and P-cadherin. When the expression of cN390 delta was induced by Zn2+, cadherin-dependent adhesion of the transfected cells was inhibited, resulting in the dispersion of cell colonies, although their contacts were maintained under high cell density conditions. In these cultures, cN390 delta was expressed not only on the free surfaces of the cells but also at cell- cell junctions. The endogenous cadherins were concentrated at cell-cell junctions under normal conditions. As a result of cN390 delta expression, however, the endogenous cadherins localizing at the cell- cell junctions were largely diminished, suggesting that these molecules were replaced by the mutant molecules at these sites. As a control, we transfected the same cell line with cDNA of a truncated form of N- cadherin cadherin whose intracellular C terminus had been deleted leaving the extracellular domain intact. This molecule had no effect on cell-cell adhesion, nor did it localize to cell-cell contact sites. We also found that the association of the endogenous cadherins with alpha- and beta-catenins and plakoglobin was not affected by the expression of cN390 delta, which also formed a complex with these molecules, suggesting that no competition occurred between the endogenous and exogenous cadherins for these cytoplasmic proteins. These and other additional results suggest that the nonfunctional cadherins whose intracellular domain is intact occupy the sites where the endogenous cadherins should localize, through interactions with the cytoskeleton, and inhibit the cadherin adhesion system.
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|
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|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
S. Norvell and K. Green Contributions of extracellular and intracellular domains of full length and chimeric cadherin molecules to junction assembly in epithelial cells J. Cell Sci., January 5, 1998; 111(9): 1305 - 1318. [Abstract] [PDF]
|
|
|
|
|
|
C Marcozzi, I. Burdett, R. Buxton, and A. Magee Coexpression of both types of desmosomal cadherin and plakoglobin confers strong intercellular adhesion J. Cell Sci., January 2, 1998; 111(4): 495 - 509. [Abstract] [PDF]
|
|
|
|
|
|
A Espeseth, G Marnellos, and C Kintner The role of F-cadherin in localizing cells during neural tube formation in Xenopus embryos Development, January 1, 1998; 125(2): 301 - 312. [Abstract] [PDF]
|
|
|
|
|
|
J. E. Lewis, J. K. Wahl III, K. M. Sass, P. J. Jensen, K. R. Johnson, and M. J. Wheelock Cross-Talk between Adherens Junctions and Desmosomes Depends on Plakoglobin J. Cell Biol., February 24, 1997; 136(4): 919 - 934. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Zhu and F. Watt Expression of a dominant negative cadherin mutant inhibits proliferation and stimulates terminal differentiation of human epidermal keratinocytes J. Cell Sci., January 12, 1996; 109(13): 3013 - 3023. [Abstract] [PDF]
|
|
|
|
|
|
R. Troyanovsky, N. Chitaev, and S. Troyanovsky Cadherin binding sites of plakoglobin: localization, specificity and role in targeting to adhering junctions J. Cell Sci., January 12, 1996; 109(13): 3069 - 3078. [Abstract] [PDF]
|
|
|
|
|
|
C. Hertig, M Eppenberger-Eberhardt, S Koch, and H. Eppenberger N-cadherin in adult rat cardiomyocytes in culture. I. Functional role of N-cadherin and impairment of cell-cell contact by a truncated N-cadherin mutant J. Cell Sci., January 1, 1996; 109(1): 1 - 10. [Abstract] [PDF]
|
|
|
|
|
|
R. B. Thomson, P. Igarashi, D. Biemesderfer, R. Kim, A. Abu-Alfa, M. Soleimani, and P. S. Aronson Isolation and cDNA Cloning of Ksp-cadherin, a Novel Kidney-specific Member of the Cadherin Multigene Family J. Biol. Chem., July 21, 1995; 270(29): 17594 - 17601. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Rubinfeld, B. Souza, I. Albert, S. Munemitsu, and P. Polakis The APC Protein and E-cadherin Form Similar but Independent Complexes with alpha-Catenin, beta-Catenin, and Plakoglobin J. Biol. Chem., March 10, 1995; 270(10): 5549 - 5555. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Gluck and A. Ben-Ze'ev Modulation of alpha-actinin levels affects cell motility and confers tumorigenicity on 3T3 cells J. Cell Sci., July 1, 1994; 107(7): 1773 - 1782. [Abstract] [PDF]
|
|
|
|
|
|
E Levine, C. Lee, C Kintner, and B. Gumbiner Selective disruption of E-cadherin function in early Xenopus embryos by a dominant negative mutant Development, January 4, 1994; 120(4): 901 - 909. [Abstract] [PDF]
|
|
|
|
 |
|
 B Rubinfeld, B Souza, I Albert, O Muller, S. Chamberlain, F. Masiarz, S Munemitsu, and P Polakis Association of the APC gene product with beta-catenin Science, December 10, 1993; 262(5140): 1731 - 1734. [Abstract] [PDF]
|
|
|
|
|
|
M. Goldberg, C. Peshkovsky, A. Shifteh, and Q. Al-Awqati {micro}-Protocadherin, a Novel Developmentally Regulated Protocadherin with Mucin-like Domains J. Biol. Chem., August 4, 2000; 275(32): 24622 - 24629. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Horikawa and M. Takeichi Requirement of the juxtamembrane domain of the cadherin cytoplasmic tail for morphogenetic cell rearrangement during myotome development J. Cell Biol., December 24, 2001; 155(7): 1297 - 1306. [Abstract] [Full Text] [PDF]
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