![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
MW Craighead, S Bowden, R Watson and J Armstrong
Imperial Cancer Research Fund, London, England.
The ypt2 gene of the fission yeast Schizosaccharomyces pombe encodes a member of the ypt/rab family of small GTP-binding proteins, related in sequence to Sec4p of Saccharomyces cerevisiae but closer to mammalian rab8. We have introduced a mutation into the gene corresponding to a mutation identified in ypt1, in which a conserved valine residue was altered to asparagine. The mutated ypt2 gene was introduced into the S. pombe genome by gene replacement. The resulting strain was temperature- sensitive for growth. Normal growth was restored by introduction of a plasmid-borne wild-type ypt2 cDNA or by cDNA for rab8 but not by various other rab or ypt sequences. At restrictive temperature the mutant cells accumulated the secretory protein acid phosphatase in a form that appeared to be fully glycosylated and acquired a population of vesicles detectable by electron microscopy. Thus the ypt2 protein, and by inference rab8, appear to function in the last stage of the secretory pathway.
This article has been cited by other articles:
|
|
 |
|
  A. Yoneda and T. L. Doering A Eukaryotic Capsular Polysaccharide Is Synthesized Intracellularly and Secreted via Exocytosis Mol. Biol. Cell, December 1, 2006; 17(12): 5131 - 5140. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Zheng, L. Camacho, E. Wee, H. Batoko, J. Legen, C. J. Leaver, R. Malho, P. J. Hussey, and I. Moore A Rab-E GTPase Mutant Acts Downstream of the Rab-D Subclass in Biosynthetic Membrane Traffic to the Plasma Membrane in Tobacco Leaf Epidermis PLANT CELL, July 1, 2005; 17(7): 2020 - 2036. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kita, R. Sugiura, H. Shoji, Y. He, L. Deng, Y. Lu, S. O. Sio, K. Takegawa, M. Sakaue, H. Shuntoh, et al. Loss of Apm1, the {micro}1 Subunit of the Clathrin-Associated Adaptor-Protein-1 Complex, Causes Distinct Phenotypes and Synthetic Lethality with Calcineurin Deletion in Fission Yeast Mol. Biol. Cell, June 1, 2004; 15(6): 2920 - 2931. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Santos, J. Gutierrez, T. M. Calonge, and P. Perez Novel Rho GTPase Involved in Cytokinesis and Cell Wall Integrity in the Fission Yeast Schizosaccharomyces pombe Eukaryot. Cell, June 1, 2003; 2(3): 521 - 533. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Matynia, S. S. Salus, and S. Sazer Three proteins required for early steps in the protein secretory pathway also affect nuclear envelope structure and cell cycle progression in fission yeast J. Cell Sci., January 15, 2002; 115(2): 421 - 431. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Jin and D. C. Amberg Fission Yeast Aip3p (spAip3p) Is Required for an Alternative Actin-directed Polarity Program Mol. Biol. Cell, May 1, 2001; 12(5): 1275 - 1291. [Abstract] [Full Text]
|
|
|
|
|
|
P Martinez and P. Ljungdahl The SHR3 homologue from S. pombe demonstrates a conserved function of ER packaging chaperones J. Cell Sci., January 12, 2000; 113(23): 4351 - 4362. [Abstract] [PDF]
|
|
|
|
|
|
N. H. Battey, N. C. James, A. J. Greenland, and C. Brownlee Exocytosis and Endocytosis PLANT CELL, April 1, 1999; 11(4): 643 - 660. [Full Text]
|
|
|
|
|
|
D Deretic, L. Huber, N Ransom, M Mancini, K Simons, and D. Papermaster rab8 in retinal photoreceptors may participate in rhodopsin transport and in rod outer segment disk morphogenesis J. Cell Sci., January 1, 1995; 108(1): 215 - 224. [Abstract] [PDF]
|
|
|
|
|
|
H. Wang, X. Tang, J. Liu, S. Trautmann, D. Balasundaram, D. McCollum, and M. K. Balasubramanian The Multiprotein Exocyst Complex Is Essential for Cell Separation in Schizosaccharomyces pombe Mol. Biol. Cell, February 1, 2002; 13(2): 515 - 529. [Abstract] [Full Text] [PDF]
|
|
