![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
JJ Mulero and TD Fox
Sections of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853-2703.
The ability to replace wild-type mitochondrial DNA sequences in yeast with in vitro-generated mutations has been exploited to study the mechanism by which the nuclearly encoded PET111 protein specifically activates translation of the mitochondrially coded COX2 mRNA. We have generated three mutations in vitro that alter the COX2 mRNA 5'- untranslated leader (UTL) and introduced them into the mitochondrial genome, replacing the wild-type sequence. None of the mutations significantly affected the steady-state level of COX2 mRNA. Deletion of a single base at position -24 (relative to the translation initiation codon) in the 5'-UTL (cox2-11) reduced COX2 mRNA translation and respiratory growth, whereas insertion of four bases in place of the deleted base (cox2-12) and deletion of bases -30 to -2 (cox2-13) completely blocked both. Six spontaneous nuclear mutations were selected as suppressors of the single-base 5'-UTL deletion, cox2-11. One of these mapped to PET111 and was shown to be a missense mutation that changed residue 652 from Ala to Thr. This suppressor, PET111-20, failed to suppress the 29-base deletion, cox2-13, but very weakly suppressed the insertion mutation, cox2-12. PET111-20 also enhanced translation of a partially functional COX2 mRNA with a wild-type 5'-UTL but a mutant initiation codon. Although overexpression of the wild-type PET111 protein caused weak suppression of the single-base deletion, cox2-11, the PET111-20 suppressor mutation did not function simply by increasing the level of the protein.(ABSTRACT TRUNCATED AT 250 WORDS)
This article has been cited by other articles:
|
|
 |
|
  O. Khalimonchuk, A. Bird, and D. R. Winge Evidence for a Pro-oxidant Intermediate in the Assembly of Cytochrome Oxidase J. Biol. Chem., June 15, 2007; 282(24): 17442 - 17449. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Fontanesi, I. C. Soto, D. Horn, and A. Barrientos Assembly of mitochondrial cytochrome c-oxidase, a complicated and highly regulated cellular process Am J Physiol Cell Physiol, December 1, 2006; 291(6): C1129 - C1147. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. H. Williams, N. Bsat, N. Bonnefoy, C. A. Butler, and T. D. Fox Alteration of a Novel Dispensable Mitochondrial Ribosomal Small-Subunit Protein, Rsm28p, Allows Translation of Defective COX2 mRNAs Eukaryot. Cell, February 1, 2005; 4(2): 337 - 345. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. H. Williams, X. Perez-Martinez, and T. D. Fox MrpL36p, a Highly Diverged L31 Ribosomal Protein Homolog With Additional Functional Domains in Saccharomyces cerevisiae Mitochondria Genetics, May 1, 2004; 167(1): 65 - 75. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Demlow and T. D. Fox Activity of Mitochondrially Synthesized Reporter Proteins Is Lower Than That of Imported Proteins and Is Increased by Lowering cAMP in Glucose-Grown Saccharomyces cerevisiae Cells Genetics, November 1, 2003; 165(3): 961 - 974. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Golik, N. Bonnefoy, T. Szczepanek, Y. Saint-Georges, and J. Lazowska The Rieske FeS protein encoded and synthesized within mitochondria complements a deficiency in the nuclear gene PNAS, July 22, 2003; 100(15): 8844 - 8849. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. H. WILLIAMS and T. D. FOX Antagonistic signals within the COX2 mRNA coding sequence control its translation in Saccharomyces cerevisiae mitochondria RNA, April 1, 2003; 9(4): 419 - 431. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Naithani, S. A. Saracco, C. A. Butler, and T. D. Fox Interactions among COX1, COX2, and COX3 mRNA-specific Translational Activator Proteins on the Inner Surface of the Mitochondrial Inner Membrane of Saccharomyces cerevisiae Mol. Biol. Cell, January 1, 2003; 14(1): 324 - 333. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. A. Broadley, C. M. Demlow, and T. D. Fox Peripheral Mitochondrial Inner Membrane Protein, Mss2p, Required for Export of the Mitochondrially Coded Cox2p C Tail in Saccharomyces cerevisiae Mol. Cell. Biol., November 15, 2001; 21(22): 7663 - 7672. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Bonnefoy, N. Bsat, and T. D. Fox Mitochondrial Translation of Saccharomyces cerevisiae COX2 mRNA Is Controlled by the Nucleotide Sequence Specifying the Pre-Cox2p Leader Peptide Mol. Cell. Biol., April 1, 2001; 21(7): 2359 - 2372. [Abstract] [Full Text]
|
|
|
|
 |
|
 O. A. Kolesnikova, N. S. Entelis, H. Mireau, T. D. Fox, R. P. Martin, and I. A. Tarassov Suppression of Mutations in Mitochondrial DNA by tRNAs Imported from the Cytoplasm Science, September 15, 2000; 289(5486): 1931 - 1933. [Abstract] [Full Text]
|
|
|
|
|
|
H. Mireau, A. Cosset, L. Marechal-Drouard, T. D. Fox, I. D. Small, and A. Dietrich Expression of Arabidopsis thaliana Mitochondrial Alanyl-tRNA Synthetase Is Not Sufficient to Trigger Mitochondrial Import of tRNAAla in Yeast J. Biol. Chem., April 28, 2000; 275(18): 13291 - 13296. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Costanzo, N. Bonnefoy, E. H. Williams, G. D. Clark-Walker, and T. D. Fox Highly Diverged Homologs of Saccharomyces cerevisiae Mitochondrial mRNA-Specific Translational Activators Have Orthologous Functions in Other Budding Yeasts Genetics, March 1, 2000; 154(3): 999 - 1012. [Abstract] [Full Text]
|
|
|
|
|
|
S. He and T. D. Fox Mutations Affecting a Yeast Mitochondrial Inner Membrane Protein, Pnt1p, Block Export of a Mitochondrially Synthesized Fusion Protein from the Matrix Mol. Cell. Biol., October 1, 1999; 19(10): 6598 - 6607. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. P. Ellis, H. B. Lukins, P. Nagley, and B. E. Corner Suppression of a Nuclear aep2 Mutation in Saccharomyces cerevisiae by a Base Substitution in the 5'-Untranslated Region of the Mitochondrial oli1 Gene Encoding Subunit 9 of ATP Synthase Genetics, April 1, 1999; 151(4): 1353 - 1363. [Abstract] [Full Text]
|
|
|
|
|
|
N. S. Green-Willms, T. D. Fox, and M. C. Costanzo Functional Interactions between Yeast Mitochondrial Ribosomes and mRNA 5' Untranslated Leaders Mol. Cell. Biol., April 1, 1998; 18(4): 1826 - 1834. [Abstract] [Full Text]
|
|
|
|
|
|
N. S. Green-Willms, C. A. Butler, H. M. Dunstan, and T. D. Fox Pet111p, an Inner Membrane-bound Translational Activator That Limits Expression of the Saccharomyces cerevisiae Mitochondrial Gene COX2 J. Biol. Chem., February 23, 2001; 276(9): 6392 - 6397. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Saracco and T. D. Fox Cox18p Is Required for Export of the Mitochondrially Encoded Saccharomyces cerevisiae Cox2p C-Tail and Interacts with Pnt1p and Mss2p in the Inner Membrane Mol. Biol. Cell, April 1, 2002; 13(4): 1122 - 1131. [Abstract] [Full Text] [PDF]
|
|
