![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
IB Bezprozvanny, K Ondrias, E Kaftan, DA Stoyanovsky and BE Ehrlich
Department of Medicine, University of Connecticut Health Center, Farmington 06030.
Heparin has been used as a potent competitive inhibitor of inositol 1,4,5-trisphosphate (IP3)-binding to IP3 receptors and to block IP3- gated calcium channels in bilayer experiments. In contrast to the effect on the IP3-gated channel, heparin (0.1-1 micrograms/ml) opened the Ca release channel (ryanodine receptor). Other polyanions such as pentosan polysulfate and polyvinyl sulfate also activated the Ca release channel. The effect of polyanions on the Ca release channel was Ca dependent. Polyanion addition activated the Ca release channel when free Ca was > 80 nM, but was ineffective when free Ca was < 20 nM. The level of channel activation could be altered by manipulating the free Ca concentration. These results suggest that the polyanions act by increasing the local concentration of Ca near regulatory sites on the channel complex. As most cells have both types of intracellular channels, the opposite effects of the polyanions on the two channel types suggests that addition of polyanions to intact cells may produce multiple effects.
This article has been cited by other articles:
|
|
 |
|
  L. F. Horowitz, W. Hirdes, B.-C. Suh, D. W. Hilgemann, K. Mackie, and B. Hille Phospholipase C in Living Cells: Activation, Inhibition, Ca2+ Requirement, and Regulation of M Current J. Gen. Physiol., August 29, 2005; 126(3): 243 - 262. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Dammermann and A. H. Guse Functional Ryanodine Receptor Expression Is Required for NAADP-mediated Local Ca2+ Signaling in T-lymphocytes J. Biol. Chem., June 3, 2005; 280(22): 21394 - 21399. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Verkhratsky Physiology and Pathophysiology of the Calcium Store in the Endoplasmic Reticulum of Neurons Physiol Rev, January 1, 2005; 85(1): 201 - 279. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Laporte, A. Hui, and I. Laher Pharmacological Modulation of Sarcoplasmic Reticulum Function in Smooth Muscle Pharmacol. Rev., December 1, 2004; 56(4): 439 - 513. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. A. Schrader, S. P. Perrett, L. Ye, and M. J. Friedlander Substrates for Coincidence Detection and Calcium Signaling for Induction of Synaptic Potentiation in the Neonatal Visual Cortex J Neurophysiol, June 1, 2004; 91(6): 2747 - 2764. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. J. Jenniskens, M. Ringvall, W. J. H. Koopman, J. Ledin, L. Kjellen, P. H. G. M. Willems, E. Forsberg, J. H. Veerkamp, and T. H. van Kuppevelt Disturbed Ca2+ kinetics in N-deacetylase/N-sulfotransferase-1 defective myotubes J. Cell Sci., June 1, 2003; 116(11): 2187 - 2193. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. E. Hoesch, K. Yienger, D. Weinreich, and J. P. Y. Kao Coexistence of Functional IP3 and Ryanodine Receptors in Vagal Sensory Neurons and Their Activation by ATP J Neurophysiol, September 1, 2002; 88(3): 1212 - 1219. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Koulen and B. E. Ehrlich Reversible Block of the Calcium Release Channel/Ryanodine Receptor by Protamine, a Heparin Antidote Mol. Biol. Cell, July 1, 2000; 11(7): 2213 - 2219. [Abstract] [Full Text]
|
|
|
|
 |
|
 P. C. Kouretas, Y. D. Kim, P. A. Cahill, A. K. Myers, L. N. To, Y.-N. Wang, J. V. Sitzmann, and R. L. Hannan Nonanticoagulant Heparin Prevents Coronary Endothelial Dysfunction After Brief Ischemia-Reperfusion Injury in the Dog Circulation, March 2, 1999; 99(8): 1062 - 1068. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Saeki, J.-B. Shen, and A. J. Pappano Inositol-1,4,5-trisphosphate increases contractions but not L-type calcium current in guinea pig ventricular myocytes Cardiovasc Res, March 1, 1999; 41(3): 620 - 628. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Narasimhan, I. N. Pessah, and D. J. Linden Inositol-1,4,5-Trisphosphate Receptor-Mediated Ca Mobilization Is Not Required for Cerebellar Long-Term Depression in Reduced Preparations J Neurophysiol, December 1, 1998; 80(6): 2963 - 2974. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Cruzblanca, D.-S. Koh, and B. Hille Bradykinin inhibits M current via phospholipase C and Ca2+ release from IP3-sensitive Ca2+ stores in rat sympathetic neurons PNAS, June 9, 1998; 95(12): 7151 - 7156. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J. Carroll, C. S. Ramarao, L. M. Mehlmann, S. Roche, M. Terasaki, and L. A. Jaffe Calcium Release at Fertilization in Starfish Eggs Is Mediated by Phospholipase Cgamma J. Cell Biol., September 22, 1997; 138(6): 1303 - 1311. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Zucchi and S. Ronca-Testoni The Sarcoplasmic Reticulum Ca2+ Channel/Ryanodine Receptor: Modulation by Endogenous Effectors, Drugs and Disease States Pharmacol. Rev., March 1, 1997; 49(1): 1 - 52. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Willmott, J. K. Sethi, T. F. Walseth, H. C. Lee, A. M. White, and A. Galione Nitric Oxide-induced Mobilization of Intracellular Calcium via the Cyclic ADP-ribose Signaling Pathway J. Biol. Chem., February 16, 1996; 271(7): 3699 - 3705. [Abstract] [Full Text] [PDF]
|
|
