![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
TC Taylor, M Kanstein, P Weidman and P Melancon
Department of Chemistry and Biochemistry, University of Colorado, Boulder 80303-0215.
We investigated the role of ADP-ribosylation factors (ARFs) in Golgi function using biochemical and morphological cell-free assays. An ARF- free cytosol produced from soluble Chinese hamster ovary (CHO) extracts supports intra-Golgi transport by a mechanism that is biochemically indistinguishable from control transport reactions: ARF-free transport reactions are NSF-dependent, remain sensitive to the donor Golgi- specific inhibitor ilimaquinone, and exhibit kinetics that are identical to that of control reactions containing ARFs. In contrast, ARF-free cytosol does not support the formation of coated vesicles on Golgi cisternae. However, vesicle formation is reconstituted upon the addition of ARF1. These data suggest that neither soluble ARFs nor coated vesicle formation are essential for transport. We conclude that cell-free intra-Golgi transport proceeds via a coated vesicle- independent mechanism regardless of vesicle formation on Golgi cisternae.
This article has been cited by other articles:
|
|
 |
|
  G. A. Belov, M. H. Fogg, and E. Ehrenfeld Poliovirus Proteins Induce Membrane Association of GTPase ADP-Ribosylation Factor J. Virol., June 1, 2005; 79(11): 7207 - 7216. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Stuven, A. Porat, F. Shimron, E. Fass, D. Kaloyanova, B. Brugger, F. T. Wieland, Z. Elazar, and J. B. Helms Intra-Golgi Protein Transport Depends on a Cholesterol Balance in the Lipid Membrane J. Biol. Chem., December 26, 2003; 278(52): 53112 - 53122. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. K. Pullikuth and P. J. Weidman In Vitro Transport on Cis and Trans Sides of the Golgi Involves Two Distinct Types of Coatomer and ADP-ribosylation Factor-independent Transport Intermediates J. Biol. Chem., December 20, 2002; 277(52): 50355 - 50364. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Porat, Y. Sagiv, and Z. Elazar A 56-kDa Selenium-binding Protein Participates in Intra-Golgi Protein Transport J. Biol. Chem., May 5, 2000; 275(19): 14457 - 14465. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-C. Lin, H. D. Love, J. N. Gushue, J. J.M. Bergeron, and J. Ostermann ER/Golgi Intermediates Acquire Golgi Enzymes by Brefeldin A-Sensitive Retrograde Transport In Vitro J. Cell Biol., December 27, 1999; 147(7): 1457 - 1472. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Lavoie, J. Paiement, M. Dominguez, L. Roy, S. Dahan, J.N. Gushue, and J.J.M. Bergeron Roles for {alpha}2p24 and COPI in Endoplasmic Reticulum Cargo Exit Site Formation J. Cell Biol., July 26, 1999; 146(2): 285 - 300. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Dominguez, A. Fazel, S. Dahan, J. Lovell, L. Hermo, A. Claude, P. Melancon, and J.J.M. Bergeron Fusogenic Domains of Golgi Membranes Are Sequestered into Specialized Regions of the Stack that Can Be Released by Mechanical Fragmentation J. Cell Biol., May 17, 1999; 145(4): 673 - 688. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Jones, D. Spiro, T Kirchhausen, P Melancon, and M Wessling-Resnick Studies on the inhibition of endosome fusion by GTPgammaS-bound ARF J. Cell Sci., January 10, 1999; 112(20): 3477 - 3485. [Abstract] [PDF]
|
|
|
|
|
|
D. M. Walter, K. S. Paul, and M. G. Waters Purification and Characterization of a Novel 13 S Hetero-oligomeric Protein Complex That Stimulates in Vitro Golgi Transport J. Biol. Chem., November 6, 1998; 273(45): 29565 - 29576. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Godi, I. Santone, P. Pertile, P. Devarajan, P. R. Stabach, J. S. Morrow, G. Di Tullio, R. Polishchuk, T. C. Petrucci, A. Luini, et al. ADP ribosylation factor regulates spectrin binding to the Golgi complex PNAS, July 21, 1998; 95(15): 8607 - 8612. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Happe and P. Weidman Cell-free Transport to Distinct Golgi Cisternae Is Compartment Specific and ARF Independent J. Cell Biol., February 9, 1998; 140(3): 511 - 523. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. D. Love, C.-C. Lin, C. S. Short, and J. Ostermann Isolation of Functional Golgi-derived Vesicles with a Possible Role in Retrograde Transport J. Cell Biol., February 9, 1998; 140(3): 541 - 551. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Mironov JR., A. Luini, and A. Mironov A synthetic model of intra-Golgi traffic FASEB J, February 1, 1998; 12(2): 249 - 252. [Abstract] [Full Text]
|
|
|
|
|
|
A. A. Mironov, P. Weidman, and A. Luini Variations on the Intracellular Transport Theme: Maturing Cisternae and Trafficking Tubules J. Cell Biol., August 11, 1997; 138(3): 481 - 484. [Full Text] [PDF]
|
|
|
|
|
|
D. J. Spiro, T. C. Taylor, P. Melanon, and M. Wessling-Resnick Cytosolic ADP-ribosylation Factors Are Not Required for Endosome-Endosome Fusion but Are Necessary for GTP[IMAGE]S Inhibition of Fusion J. Biol. Chem., June 9, 1995; 270(23): 13693 - 13697. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Traub, S. Kornfeld, and E. Ungewickell Different Domains of the AP-1 Adaptor Complex Are Required for Golgi Membrane Binding and Clathrin Recruitment J. Biol. Chem., March 3, 1995; 270(9): 4933 - 4942. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Porat and Z. Elazar Regulation of Intra-Golgi Membrane Transport by Calcium J. Biol. Chem., September 15, 2000; 275(38): 29233 - 29237. [Abstract] [Full Text] [PDF]
|
|
