![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
LS Huang, P Tzou and PW Sternberg
Howard Hughes Medical Institute, Division of Biology, California Institute of Technology, Pasadena 91125.
During Caenorhabditis elegans vulval development, an inductive signal from the anchor cell stimulates three of the six vulval precursor cells (VPCs) to adopt vulval rather than nonvulval epidermal fates. Genes necessary for this induction include the lin-3 growth factor, the let- 23 receptor tyrosine kinase, and let-60 ras. lin-15 is a negative regulator of this inductive pathway. In lin-15 mutant animals, all six VPCs adopt vulval fates, even in the absence of inductive signal. Previous genetic studies suggested that lin-15 is a complex locus with two independently mutable activities, A and B. We have cloned the lin- 15 locus by germline transformation and find that it encodes two nonoverlapping transcripts that are transcribed in the same direction. The downstream transcript encodes the lin-15A function; the upstream transcript encodes the lin-15B function. The predicted lin-15A and lin- 15B proteins are novel and hydrophilic. We have identified a molecular null allele of lin-15 and have used it to analyze the role of lin-15 in the signaling pathway. We find that lin-15 acts upstream of let-23 and in parallel to the inductive signal.
This article has been cited by other articles:
|
|
 |
|
  M. M. Harrison, X. Lu, and H. R. Horvitz LIN-61, One of Two Caenorhabditis elegans Malignant-Brain-Tumor-Repeat-Containing Proteins, Acts With the DRM and NuRD-Like Protein Complexes in Vulval Development but Not in Certain Other Biological Processes Genetics, May 1, 2007; 176(1): 255 - 271. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Hornsten, J. Lieberthal, S. Fadia, R. Malins, L. Ha, X. Xu, I. Daigle, M. Markowitz, G. O'Connor, R. Plasterk, et al. APL-1, a Caenorhabditis elegans protein related to the human beta-amyloid precursor protein, is essential for viability PNAS, February 6, 2007; 104(6): 1971 - 1976. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Harris, M. Lowden, I. Clejan, M. Tzoneva, J. H. Thomas, J. Hodgkin, and S. Ahmed Mutator Phenotype of Caenorhabditis elegans DNA Damage Checkpoint Mutants Genetics, October 1, 2006; 174(2): 601 - 616. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. C. Andersen, X. Lu, and H. R. Horvitz C. elegans ISWI and NURF301 antagonize an Rb-like pathway in the determination of multiple cell fates Development, July 15, 2006; 133(14): 2695 - 2704. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. Ceol, F. Stegmeier, M. M. Harrison, and H. R. Horvitz Identification and Classification of Genes That Act Antagonistically to let-60 Ras Signaling in Caenorhabditis elegans Vulval Development Genetics, June 1, 2006; 173(2): 709 - 726. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Goldstein, D. Glossip, S. Nayak, and K. Kornfeld The CRAL/TRIO and GOLD Domain Protein CGR-1 Promotes Induction of Vulval Cell Fates in Caenorhabditis elegans and Interacts Genetically With the Ras Signaling Pathway Genetics, February 1, 2006; 172(2): 929 - 942. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Chesney, A. R. Kidd III, and J. Kimble gon-14 Functions With Class B and Class C Synthetic Multivulva Genes to Control Larval Growth in Caenorhabditis elegans Genetics, February 1, 2006; 172(2): 915 - 928. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. Davison, M. M. Harrison, A. J. M. Walhout, M. Vidal, and H. R. Horvitz lin-8, Which Antagonizes Caenorhabditis elegans Ras-Mediated Vulval Induction, Encodes a Novel Nuclear Protein That Interacts With the LIN-35 Rb Protein Genetics, November 1, 2005; 171(3): 1017 - 1031. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Clouaire, M. Roussigne, V. Ecochard, C. Mathe, F. Amalric, and J.-P. Girard The THAP domain of THAP1 is a large C2CH module with zinc-dependent sequence-specific DNA-binding activity PNAS, May 10, 2005; 102(19): 6907 - 6912. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. M. Hajdu-Cronin, W. J. Chen, and P. W. Sternberg The L-Type Cyclin CYL-1 and the Heat-Shock-Factor HSF-1 Are Required for Heat-Shock-Induced Protein Expression in Caenorhabditis elegans Genetics, December 1, 2004; 168(4): 1937 - 1949. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Cui, D. S. Fay, and M. Han lin-35/Rb Cooperates With the SWI/SNF Complex to Control Caenorhabditis elegans Larval Development Genetics, July 1, 2004; 167(3): 1177 - 1185. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Garbe, J. B. Doto, and M. V. Sundaram Caenorhabditis elegans lin-35/Rb, efl-1/E2F and Other Synthetic Multivulva Genes Negatively Regulate the Anaphase-Promoting Complex Gene mat-3/APC8 Genetics, June 1, 2004; 167(2): 663 - 672. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ailion and J. H. Thomas Isolation and Characterization of High-Temperature-Induced Dauer Formation Mutants in Caenorhabditis elegans Genetics, September 1, 2003; 165(1): 127 - 144. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S. Fay, E. Large, M. Han, and M. Darland lin-35/Rb and ubc-18, an E2 ubiquitin-conjugating enzyme, function redundantly to control pharyngeal morphogenesis in C. elegans Development, July 15, 2003; 130(14): 3319 - 3330. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. Thomas, C. J. Ceol, H. T. Schwartz, and H. R. Horvitz New Genes That Interact With lin-35 Rb to Negatively Regulate the let-60 ras Pathway in Caenorhabditis elegans Genetics, May 1, 2003; 164(1): 135 - 151. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. Schafer, C. J. Haycraft, J. H. Thomas, B. K. Yoder, and P. Swoboda XBX-1 Encodes a Dynein Light Intermediate Chain Required for Retrograde Intraflagellar Transport and Cilia Assembly in Caenorhabditis elegans Mol. Biol. Cell, May 1, 2003; 14(5): 2057 - 2070. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Moghal and P. W. Sternberg A component of the transcriptional mediator complex inhibits RAS-dependent vulval fate specification in C. elegans Development, January 1, 2003; 130(1): 57 - 69. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Shaham and C. I. Bargmann Control of neuronal subtype identity by the C. elegans ARID protein CFI-1 Genes & Dev., April 15, 2002; 16(8): 972 - 983. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S. Fay, S. Keenan, and M. Han fzr-1 and lin-35/Rb function redundantly to control cell proliferation in C. elegans as revealed by a nonbiased synthetic screen Genes & Dev., February 15, 2002; 16(4): 503 - 517. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Chen and M. Han Role of C. elegans lin-40 MTA in vulval fate specification and morphogenesis Development, December 1, 2001; 128(23): 4911 - 4921. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. N. Nathoo, R. A. Moeller, B. A. Westlund, and A. C. Hart Identification of neuropeptide-like protein gene families in Caenorhabditiselegans and other species PNAS, November 20, 2001; 98(24): 14000 - 14005. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. T. Kunkel, D. B. Johnstone, J. H. Thomas, and L. Salkoff Mutants of a Temperature-Sensitive Two-P Domain Potassium Channel J. Neurosci., October 15, 2000; 20(20): 7517 - 7524. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Zallen, E. L. Peckol, D. M. Tobin, and C. I. Bargmann Neuronal Cell Shape and Neurite Initiation Are Regulated by the Ndr Kinase SAX-1, a Member of the Orb6/COT-1/Warts Serine/Threonine Kinase Family Mol. Biol. Cell, September 1, 2000; 11(9): 3177 - 3190. [Abstract] [Full Text]
|
|
|
|
 |
|
 E. Betran and M. Ashburner Duplication, Dicistronic Transcription, and Subsequent Evolution of the Alcohol dehydrogenase and Alcohol dehydrogenase-related Genes in Drosophila Mol. Biol. Evol., September 1, 2000; 17(9): 1344 - 1352. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Meléndez and I. Greenwald Caenorhabditis elegans lin-13, a Member of the LIN-35 Rb Class of Genes Involved in Vulval Development, Encodes a Protein With Zinc Fingers and an LXCXE Motif Genetics, July 1, 2000; 155(3): 1127 - 1137. [Abstract] [Full Text]
|
|
|
|
|
|
D. A. Birnby, E. M. Link, J. J. Vowels, H. Tian, P. L. Colacurcio, and J. H. Thomas A Transmembrane Guanylyl Cyclase (DAF-11) and Hsp90 (DAF-21) Regulate a Common Set of Chemosensory Behaviors in Caenorhabditis elegans Genetics, May 1, 2000; 155(1): 85 - 104. [Abstract] [Full Text]
|
|
|
|
|
|
M Wang and P. Sternberg Patterning of the C. elegans 1 degrees vulval lineage by RAS and Wnt pathways Development, January 12, 2000; 127(23): 5047 - 5058. [Abstract] [PDF]
|
|
|
|
|
|
T von Zelewsky, F Palladino, K Brunschwig, H Tobler, A Hajnal, and F Muller The C. elegans Mi-2 chromatin-remodelling proteins function in vulval cell fate determination Development, January 12, 2000; 127(24): 5277 - 5284. [Abstract] [PDF]
|
|
|
|
|
|
H White-Cooper, D Leroy, A MacQueen, and M. Fuller Transcription of meiotic cell cycle and terminal differentiation genes depends on a conserved chromatin associated protein, whose nuclear localisation is regulated Development, January 12, 2000; 127(24): 5463 - 5473. [Abstract] [PDF]
|
|
|
|
 |
|
 A. J. Walhout, R. Sordella, X. Lu, J. L. Hartley, G. F. Temple, M. A. Brasch, N. Thierry-Mieg, and M. Vidal Protein Interaction Mapping in C. elegans Using Proteins Involved in Vulval Development Science, January 7, 2000; 287(5450): 116 - 122. [Abstract] [Full Text]
|
|
|
|
|
|
C. Darby, C. L. Cosma, J. H. Thomas, and C. Manoil Lethal paralysis of Caenorhabditis elegans by Pseudomonas aeruginosa PNAS, December 21, 1999; 96(26): 15202 - 15207. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Hsieh, J. Liu, S. A. Kostas, C. Chang, P. W. Sternberg, and A. Fire The RING finger/B-Box factor TAM-1 and a retinoblastoma-like protein LIN-35 modulate context-dependent gene silencing in Caenorhabditis elegans Genes & Dev., November 15, 1999; 13(22): 2958 - 2970. [Abstract] [Full Text]
|
|
|
|
|
|
J. M. Bosher, P. Dufourcq, S. Sookhareea, and M. Labouesse RNA Interference Can Target Pre-mRNA: Consequences for Gene Expression in a Caenorhabditis elegans Operon Genetics, November 1, 1999; 153(3): 1245 - 1256. [Abstract] [Full Text]
|
|
|
|
|
|
A. G. Davies, C. A. Spike, J. E. Shaw, and R. K. Herman Functional Overlap Between the mec-8 Gene and Five sym Genes in Caenorhabditis elegans Genetics, September 1, 1999; 153(1): 117 - 134. [Abstract] [Full Text]
|
|
|
|
|
|
Y. M. Hajdu-Cronin, W. J. Chen, G. Patikoglou, M. R. Koelle, and P. W. Sternberg Antagonism between Goalpha and Gqalpha in Caenorhabditis elegans: the RGS protein EAT-16 is necessary for Goalpha signaling and regulates Gqalpha activity Genes & Dev., July 15, 1999; 13(14): 1780 - 1793. [Abstract] [Full Text]
|
|
|
|
|
|
M. Ailion, T. Inoue, C. I. Weaver, R. W. Holdcraft, and J. H. Thomas Neurosecretory control of aging in Caenorhabditis elegans PNAS, June 22, 1999; 96(13): 7394 - 7397. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Thomas and H. Horvitz The C. elegans gene lin-36 acts cell autonomously in the lin-35 Rb pathway Development, January 8, 1999; 126(15): 3449 - 3459. [Abstract] [PDF]
|
|
|
|
|
|
J. Zallen, S. Kirch, and C. Bargmann Genes required for axon pathfinding and extension in the C. elegans nerve ring Development, January 8, 1999; 126(16): 3679 - 3692. [Abstract] [PDF]
|
|
|
|
|
|
E. Peckol, J. Zallen, J. Yarrow, and C. Bargmann Sensory activity affects sensory axon development in C. elegans Development, January 5, 1999; 126(9): 1891 - 1902. [Abstract] [PDF]
|
|
|
|
|
|
K Morita, K. Chow, and N Ueno Regulation of body length and male tail ray pattern formation of Caenorhabditis elegans by a member of TGF-beta family Development, January 3, 1999; 126(6): 1337 - 1347. [Abstract] [PDF]
|
|
|
|
|
|
R. Y. N. Lee, E. R. Sawin, M. Chalfie, H. R. Horvitz, and L. Avery EAT-4, a Homolog of a Mammalian Sodium-Dependent Inorganic Phosphate Cotransporter, Is Necessary for Glutamatergic Neurotransmission in Caenorhabditis elegans J. Neurosci., January 1, 1999; 19(1): 159 - 167. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Treinin, B. Gillo, L. Liebman, and M. Chalfie Two functionally dependent acetylcholine subunits are encoded in a single Caenorhabditis elegans operon PNAS, December 22, 1998; 95(26): 15492 - 15495. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. K. Montgomery, S. Xu, and A. Fire RNA as a target of double-stranded RNA-mediated genetic interference in Caenorhabditis elegans PNAS, December 22, 1998; 95(26): 15502 - 15507. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Lackner and S. K. Kim Genetic Analysis of the Caenorhabditis elegans MAP Kinase Gene mpk-1 Genetics, September 1, 1998; 150(1): 103 - 117. [Abstract] [Full Text]
|
|
|
|
 |
|
 C. Serra-Pages, Q. G. Medley, M. Tang, A. Hart, and M. Streuli Liprins, a Family of LAR Transmembrane Protein-tyrosine Phosphatase-interacting Proteins J. Biol. Chem., June 19, 1998; 273(25): 15611 - 15620. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Berger, A. C. Hart, and J. M. Kaplan Galpha s-Induced Neurodegeneration in Caenorhabditis elegans J. Neurosci., April 15, 1998; 18(8): 2871 - 2880. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Gutch, A. J. Flint, J. Keller, N. K. Tonks, and M. O. Hengartner The Caenorhabditis elegans SH2 domain-containing protein tyrosine phosphatase PTP-2 participates in signal transduction during oogenesis and vulval development Genes & Dev., February 15, 1998; 12(4): 571 - 585. [Abstract] [Full Text]
|
|
|
|
|
|
L. Jiang and P. Sternberg Interactions of EGF, Wnt and HOM-C genes specify the P12 neuroectoblast fate in C. elegans Development, January 6, 1998; 125(12): 2337 - 2347. [Abstract] [PDF]
|
|
|
|
|
|
J. Maloof and C Kenyon The Hox gene lin-39 is required during C. elegans vulval induction to select the outcome of Ras signaling Development, January 1, 1998; 125(2): 181 - 190. [Abstract] [PDF]
|
|
|
|
|
|
Identification of Caenorhabditis elegans Genes Required for Neuronal Differentiation and Migration Genetics, January 1, 1998; 148(1): 151 - 166.
|
|
|
|
|
|
A. Hajnal, C. W. Whitfield, and S. K. Kim Inhibition of Caenorhabditis elegans vulval induction by gap-1 and by let-23 receptor tyrosine kinase Genes & Dev., October 15, 1997; 11(20): 2715 - 2728. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Evans, D. Zorio, M. MacMorris, C. E. Winter, K. Lea, and T. Blumenthal Operons and SL2 trans-splicing exist in nematodes outside the genus Caenorhabditis PNAS, September 2, 1997; 94(18): 9751 - 9756. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K Ogura, M Shirakawa, T M Barnes, S Hekimi, and Y Ohshima The UNC-14 protein required for axonal elongation and guidance in Caenorhabditis elegans interacts with the serine/threonine kinase UNC-51. Genes & Dev., July 15, 1997; 11(14): 1801 - 1811. [Abstract] [PDF]
|
|
|
|
|
|
S. Yu, L. Avery, E. Baude, and D. L. Garbers Guanylyl cyclase expression in specific sensory neurons: A new family of chemosensory receptors PNAS, April 1, 1997; 94(7): 3384 - 3387. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M Sundaram, J Yochem, and M Han A Ras-mediated signal transduction pathway is involved in the control of sex myoblast migration in Caenorhabditis elegans Development, January 9, 1996; 122(9): 2823 - 2833. [Abstract] [PDF]
|
|
|
|
|
|
G J Beitel, S Tuck, I Greenwald, and H R Horvitz The Caenorhabditis elegans gene lin-1 encodes an ETS-domain protein and defines a branch of the vulval induction pathway. Genes & Dev., December 15, 1995; 9(24): 3149 - 3162. [Abstract] [PDF]
|
|
|
|
|
|
N Singh and M Han sur-2, a novel gene, functions late in the let-60 ras-mediated signaling pathway during Caenorhabditis elegans vulval induction. Genes & Dev., September 15, 1995; 9(18): 2251 - 2265. [Abstract] [PDF]
|
|
|
|
|
|
L Segalat, D. Elkes, and J. Kaplan Modulation of serotonin-controlled behaviors by Go in Caenorhabditis elegans Science, March 17, 1995; 267(5204): 1648 - 1651. [Abstract] [PDF]
|
|
|
|
|
|
K Kornfeld, K L Guan, and H R Horvitz The Caenorhabditis elegans gene mek-2 is required for vulval induction and encodes a protein similar to the protein kinase MEK. Genes & Dev., March 15, 1995; 9(6): 756 - 768. [Abstract] [PDF]
|
|
|
|
|
|
S Tuck and I Greenwald lin-25, a gene required for vulval induction in Caenorhabditis elegans. Genes & Dev., February 1, 1995; 9(3): 341 - 357. [Abstract] [PDF]
|
|
|
|
|
|
H. Chamberlin and P. Sternberg The lin-3/let-23 pathway mediates inductive signalling during male spicule development in Caenorhabditis elegans Development, January 10, 1994; 120(10): 2713 - 2721. [Abstract] [PDF]
|
|
