![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
M Baum, VK Ngan and L Clarke
Department of Biological Sciences, University of California, Santa Barbara 93106.
The DNA requirements for centromere function in fission yeast have been investigated using a minichromosome assay system. Critical elements of Schizosaccharomyces pombe centromeric DNA are portions of the centromeric central core and sequences within a 2.1-kilobase segment found on all three chromosomes as part of the K-type (K/K"/dg) centromeric repeat. The S. pombe centromeric central core contains DNA sequences that appear functionally redundant, and the inverted repeat motif that flanks the central core in all native fission yeast centromeres is not essential for centromere function in circular minichromosomes. Tandem copies of centromeric repeat K", in conjunction with the central core, exert an additive effect on centromere function, increasing minichromosome mitotic stability with each additional copy. Centromeric repeats B and L, however, and parts of the central core and its core-associated repeat are dispensable and cannot substitute for K- type sequences. Several specific protein binding sites have been identified within the centromeric K-type repeat, consistent with a recently proposed model for centromere/kinetochore function in S. pombe.
This article has been cited by other articles:
|
|
 |
|
  H. D. Folco, A. L. Pidoux, T. Urano, and R. C. Allshire Heterochromatin and RNAi Are Required to Establish CENP-A Chromatin at Centromeres Science, January 4, 2008; 319(5859): 94 - 97. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Baum, K. Sanyal, P. K. Mishra, N. Thaler, and J. Carbon Formation of functional centromeric chromatin is specified epigenetically in Candida albicans PNAS, October 3, 2006; 103(40): 14877 - 14882. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Sanyal, M. Baum, and J. Carbon Centromeric DNA sequences in the pathogenic yeast Candida albicans are all different and unique PNAS, August 3, 2004; 101(31): 11374 - 11379. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Suzuki, M. Nakano, N. Nozaki, S.-i. Egashira, T. Okazaki, and H. Masumoto CENP-B Interacts with CENP-C Domains Containing Mif2 Regions Responsible for Centromere Localization J. Biol. Chem., February 13, 2004; 279(7): 5934 - 5946. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. X. Zhong, J. B. Marshall, C. Topp, R. Mroczek, A. Kato, K. Nagaki, J. A. Birchler, J. Jiang, and R. K. Dawe Centromeric Retroelements and Satellites Interact with Maize Kinetochore Protein CENH3 PLANT CELL, November 1, 2002; 14(11): 2825 - 2836. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q.-W. Jin, A. L. Pidoux, C. Decker, R. C. Allshire, and U. Fleig The Mal2p Protein Is an Essential Component of the Fission Yeast Centromere Mol. Cell. Biol., October 15, 2002; 22(20): 7168 - 7183. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Volpe, C. Kidner, I. M. Hall, G. Teng, S. I. S. Grewal, and R. A. Martienssen Regulation of Heterochromatic Silencing and Histone H3 Lysine-9 Methylation by RNAi Science, September 13, 2002; 297(5588): 1833 - 1837. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. J. Reinhart and D. P. Bartel Small RNAs Correspond to Centromere Heterochromatic Repeats Science, September 13, 2002; 297(5588): 1831 - 1831. [Full Text] [PDF]
|
|
|
|
 |
|
 H. Nakagawa, J.-K. Lee, J. Hurwitz, R. C. Allshire, J.-i. Nakayama, S. I.S. Grewal, K. Tanaka, and Y. Murakami Fission yeast CENP-B homologs nucleate centromeric heterochromatin by promoting heterochromatin-specific histone tail modifications Genes & Dev., July 15, 2002; 16(14): 1766 - 1778. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. T. Irelan, G. I. Gutkin, and L. Clarke Functional Redundancies, Distinct Localizations and Interactions Among Three Fission Yeast Homologs of Centromere Protein-B Genetics, March 1, 2001; 157(3): 1191 - 1203. [Abstract] [Full Text]
|
|
|
|
|
|
N. Ayoub, I. Goldshmidt, R. Lyakhovetsky, and A. Cohen A Fission Yeast Repression Element Cooperates With Centromere-like Sequences and Defines a mat Silent Domain Boundary Genetics, November 1, 2000; 156(3): 983 - 994. [Abstract] [Full Text]
|
|
|
|
|
|
K. Yoda, S. Ando, S. Morishita, K. Houmura, K. Hashimoto, K. Takeyasu, and T. Okazaki Human centromere protein A (CENP-A) can replace histone H3 in nucleosome reconstitution in vitro PNAS, June 6, 2000; (2000) 130189697. [Abstract] [Full Text]
|
|
|
|
|
|
M. Baum and L. Clarke Fission Yeast Homologs of Human CENP-B Have Redundant Functions Affecting Cell Growth and Chromosome Segregation Mol. Cell. Biol., April 15, 2000; 20(8): 2852 - 2864. [Abstract] [Full Text]
|
|
|
|
|
|
J. F. Partridge, B. Borgstrøm, and R. C. Allshire Distinct protein interaction domains and protein spreading in a complex centromere Genes & Dev., April 1, 2000; 14(7): 783 - 791. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. Koch Neocentromeres and alpha satellite: a proposed structural code for functional human centromere DNA Hum. Mol. Genet., January 22, 2000; 9(2): 149 - 154. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Pidoux, S Uzawa, P. Perry, W. Cande, and R. Allshire Live analysis of lagging chromosomes during anaphase and their effect on spindle elongation rate in fission yeast J. Cell Sci., January 12, 2000; 113(23): 4177 - 4191. [Abstract] [PDF]
|
|
|
|
|
|
K. Ekwall, G. Cranston, and R. C. Allshire Fission Yeast Mutants That Alleviate Transcriptional Silencing in Centromeric Flanking Repeats and Disrupt Chromosome Segregation Genetics, November 1, 1999; 153(3): 1153 - 1169. [Abstract] [Full Text]
|
|
|
|
|
|
J. T. Miller, F. Dong, S. A. Jackson, J. Song, and J. Jiang Retrotransposon-Related DNA Sequences in the Centromeres of Grass Chromosomes Genetics, December 1, 1998; 150(4): 1615 - 1623. [Abstract] [Full Text]
|
|
|
|
|
|
E. Kaszás and J. A. Birchler Meiotic Transmission Rates Correlate With Physical Features of Rearranged Centromeres in Maize Genetics, December 1, 1998; 150(4): 1683 - 1692. [Abstract] [Full Text]
|
|
|
|
|
|
F. Dong, J. T. Miller, S. A. Jackson, G.-L. Wang, P. C. Ronald, and J. Jiang Rice (Oryza sativa) centromeric regions consist of complex DNA PNAS, July 7, 1998; 95(14): 8135 - 8140. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-K. Lee, J. A. Huberman, and J. Hurwitz Purification and characterization of a CENP-B homologue protein that binds to the centromeric K-type repeat DNA of Schizosaccharomyces pombe PNAS, August 5, 1997; 94(16): 8427 - 8432. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Halverson, M. Baum, J. Stryker, J. Carbon, and L. Clarke A Centromere DNA-binding Protein from Fission Yeast Affects Chromosome Segregation and Has Homology to Human CENP-B J. Cell Biol., February 10, 1997; 136(3): 487 - 500. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T Torok, P D Harvie, M Buratovich, and P J Bryant The product of proliferation disrupter is concentrated at centromeres and required for mitotic chromosome condensation and cell proliferation in Drosophila. Genes & Dev., January 15, 1997; 11(2): 213 - 225. [Abstract] [PDF]
|
|
|
|
|
|
K Ekwall, E. Nimmo, J. Javerzat, B Borgstrom, R Egel, G Cranston, and R Allshire Mutations in the fission yeast silencing factors clr4+ and rik1+ disrupt the localisation of the chromo domain protein Swi6p and impair centromere function J. Cell Sci., January 11, 1996; 109(11): 2637 - 2648. [Abstract] [PDF]
|
|
|
|
|
|
A. F. Pluta, A. M. Mackay, A. M. Ainsztein, I. G. Goldberg, and W. C. Earnshaw The Centromere: Hub of Chromosomal Activities Science, December 8, 1995; 270(5242): 1591 - 1594. [Abstract] [PDF]
|
|
|
|
|
|
K. Yoda, S. Ando, S. Morishita, K. Houmura, K. Hashimoto, K. Takeyasu, and T. Okazaki Human centromere protein A (CENP-A) can replace histone H3 in nucleosome reconstitution in vitro PNAS, June 20, 2000; 97(13): 7266 - 7271. [Abstract] [Full Text] [PDF]
|
|
