![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
K Venstrom and L Reichardt
Department of Physiology, University of California San Francisco 94143, USA.
Integrins are major receptors used by cells to interact with extracellular matrices. In this paper, we identify the first ligands for the beta 8 family of integrins, presenting evidence that integrin heterodimers containing the beta 8 subunit mediate interactions of chick sensory neurons with laminin-1, collagen IV, and fibronectin. A polyclonal antibody, anti-beta 8-Ex, was prepared to a bacterial fusion protein expressing an extracellular portion of the chicken beta 8 subunit. In nonreducing conditions, this antibody immunoprecipitated from surface-labeled embryonic dorsal root ganglia neurons a M(r) 100 k protein, the expected M(r) of the beta 8 subunit, and putative alpha subunit(s) of M(r) 120 k. Affinity-purified anti-beta 8-Ex strongly inhibited sensory neurite outgrowth on laminin-1, collagen IV, and fibronectin-coated substrata. Binding sites were identified in a heat- resistant domain in laminin-1 and in the carboxyl terminal, 40-kDa fibronectin fragment. On substrates coated with the carboxyl terminal fragment of fibronectin, antibodies to beta 1 and beta 8 were only partially effective alone, but were completely effective in combination, at inhibiting neurite outgrowth. Results thus indicate that the integrin beta 8 subunit in association with one or more alpha subunits forms an important set of extracellular matrix receptors on sensory neurons.
This article has been cited by other articles:
|
|
 |
|
  S. Lakhe-Reddy, S. Khan, M. Konieczkowski, G. Jarad, K. L. Wu, L. F. Reichardt, Y. Takai, L. A. Bruggeman, B. Wang, J. R. Sedor, et al. beta8 Integrin Binds Rho GDP Dissociation Inhibitor-1 and Activates Rac1 to Inhibit Mesangial Cell Myofibroblast Differentiation J. Biol. Chem., July 14, 2006; 281(28): 19688 - 19699. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Proctor, K. Zang, D. Wang, R. Wang, and L. F. Reichardt Vascular Development of the Brain Requires {beta}8 Integrin Expression in the Neuroepithelium J. Neurosci., October 26, 2005; 25(43): 9940 - 9948. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K S Nair, R Naidoo, and R Chetty Expression of cell adhesion molecules in oesophageal carcinoma and its prognostic value J. Clin. Pathol., April 1, 2005; 58(4): 343 - 351. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. H. McCarty, A. Lacy-Hulbert, A. Charest, R. T. Bronson, D. Crowley, D. Housman, J. Savill, J. Roes, and R. O. Hynes Selective ablation of {alpha}v integrins in the central nervous system leads to cerebral hemorrhage, seizures, axonal degeneration and premature death Development, January 1, 2005; 132(1): 165 - 176. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Zhu, K. Motejlek, D. Wang, K. Zang, A. Schmidt, and L. F. Reichardt {beta}8 integrins are required for vascular morphogenesis in mouse embryos Development, March 8, 2003; 129(12): 2891 - 2903. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Jarad, B. Wang, S. Khan, J. DeVore, H. Miao, K. Wu, S. L. Nishimura, B. A. Wible, M. Konieczkowski, J. R. Sedor, et al. Fas Activation Induces Renal Tubular Epithelial Cell beta 8 Integrin Expression and Function in the Absence of Apoptosis J. Biol. Chem., November 27, 2002; 277(49): 47826 - 47833. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S Testaz, M Delannet, and J Duband Adhesion and migration of avian neural crest cells on fibronectin require the cooperating activities of multiple integrins of the (beta)1 and (beta)3 families J. Cell Sci., January 12, 1999; 112(24): 4715 - 4728. [Abstract] [PDF]
|
|
|
|
 |
|
 P. M. Yip, X. Zhao, A. M.P. Montgomery, and C.-H. Siu The Arg-Gly-Asp Motif in the Cell Adhesion Molecule L1 Promotes Neurite Outgrowth via Interaction with the alpha vbeta 3 Integrin Mol. Biol. Cell, February 1, 1998; 9(2): 277 - 290. [Abstract] [Full Text]
|
|
|
|
|
|
T. Jacques, J. Relvas, S Nishimura, R Pytela, G. Edwards, C. Streuli, and C ffrench-Constant Neural precursor cell chain migration and division are regulated through different beta1 integrins Development, January 8, 1998; 125(16): 3167 - 3177. [Abstract] [PDF]
|
|
|
|
|
|
N Desban and J. Duband Avian neural crest cell migration on laminin: interaction of the alpha1beta1 integrin with distinct laminin-1 domains mediates different adhesive responses J. Cell Sci., January 11, 1997; 110(21): 2729 - 2744. [Abstract] [PDF]
|
|
