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PB Meluh and D Koshland
Carnegie Institution of Washington, Department of Embryology, Baltimore, Maryland 21210, USA.
The MIF2 gene of Saccharomyces cerevisiae has been implicated in mitosis. Here we provide genetic evidence that MIF2 encodes a centromere protein. Specifically, we found that mutations in MIF2 stabilize dicentric minichromosomes and confer high instability (i.e., a synthetic acentric phenotype) to chromosomes that bear a cis-acting mutation in element I of the yeast centromeric DNA (CDEI). Similarly, we observed synthetic phenotypes between mutations in MIF2 and trans- acting mutations in three known yeast centromere protein genes- CEP1/CBF1/CPF1, NDC10/CBF2, and CEP3/CBF3B. In addition, the mif2 temperature-sensitive phenotype can be partially rescued by increased dosage of CEP1. Synthetic lethal interactions between a cep1 null mutation and mutations in either NDC10 or CEP3 were also detected. Taken together, these data suggest that the Mif2 protein interacts with Cep1p at the centromere and that the yeast centromere indeed exists as a higher order protein-DNA complex. The Mif2 and Cep1 proteins contain motifs of known transcription factors, suggesting that assembly of the yeast centromere is analogous to that of eukaryotic enhancers and origins of replication. We also show that the predicted Mif2 protein shares two short regions of homology with the mammalian centromere Ag CENP-C and that two temperature-sensitive mutations in MIF2 lie within these regions. These results provide evidence for structural conservation between yeast and mammalian centromeres.
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K. M. Hyland, J. Kingsbury, D. Koshland, and P. Hieter Ctf19p: A Novel Kinetochore Protein in Saccharomyces cerevisiae and a Potential Link between the Kinetochore and Mitotic Spindle J. Cell Biol., April 5, 1999; 145(1): 15 - 28. [Abstract] [Full Text] [PDF]
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F. G. Whitby, G. Xia, C. M. Pickart, and C. P. Hill Crystal Structure of the Human Ubiquitin-like Protein NEDD8 and Interactions with Ubiquitin Pathway Enzymes J. Biol. Chem., December 25, 1998; 273(52): 34983 - 34991. [Abstract] [Full Text] [PDF]
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T. Maney, A. W. Hunter, M. Wagenbach, and L. Wordeman Mitotic Centromere-associated Kinesin Is Important for Anaphase Chromosome Segregation J. Cell Biol., August 10, 1998; 142(3): 787 - 801. [Abstract] [Full Text] [PDF]
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T. Kamitani, K. Kito, H. P. Nguyen, T. Fukuda-Kamitani, and E. T. H. Yeh Characterization of a Second Member of the Sentrin Family of Ubiquitin-like Proteins J. Biol. Chem., May 1, 1998; 273(18): 11349 - 11353. [Abstract] [Full Text] [PDF]
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R. E. Baker, K. Harris, and K. Zhang Mutations Synthetically Lethal with cep1 Target S. cerevisiae Kinetochore Components Genetics, May 1, 1998; 149(1): 73 - 85. [Abstract] [Full Text] [PDF]
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J. P. Sanchez, Y. Murakami, J. A. Huberman, and J. Hurwitz Isolation, Characterization, and Molecular Cloning of a Protein (Abp2) That Binds to a Schizosaccharomyces pombe Origin of Replication (ars3002) Mol. Cell. Biol., March 1, 1998; 18(3): 1670 - 1681. [Abstract] [Full Text]
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M. J. Matunis, J. Wu, and G. Blobel SUMO-1 Modification and Its Role in Targeting the Ran GTPase-activating Protein, RanGAP1, to the Nuclear Pore Complex J. Cell Biol., February 9, 1998; 140(3): 499 - 509. [Abstract] [Full Text] [PDF]
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P. Kalitsis, K. J. Fowler, E. Earle, J. Hill, and K. H. A. Choo Targeted disruption of mouse centromere protein C gene leads to mitotic disarray and early embryo death PNAS, February 3, 1998; 95(3): 1136 - 1141. [Abstract] [Full Text] [PDF]
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R. Mahajan, L. Gerace, and F. Melchior Molecular Characterization of the SUMO-1 Modification of RanGAP1 and Its Role in Nuclear Envelope Association J. Cell Biol., January 26, 1998; 140(2): 259 - 270. [Abstract] [Full Text] [PDF]
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P. B. Meluh and D. Koshland Budding yeast centromere composition and assembly as revealed by in vivo cross-linking Genes & Dev., December 15, 1997; 11(24): 3401 - 3412. [Abstract] [Full Text] [PDF]
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E. S. Johnson and G. Blobel Ubc9p Is the Conjugating Enzyme for the Ubiquitin-like Protein Smt3p J. Biol. Chem., October 24, 1997; 272(43): 26799 - 26802. [Abstract] [Full Text] [PDF]
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D. A. Starr, B. C. Williams, Z. Li, B. Etemad-Moghadam, R. K. Dawe, and M. L. Goldberg Conservation of the Centromere/Kinetochore Protein ZW10 J. Cell Biol., September 22, 1997; 138(6): 1289 - 1301. [Abstract] [Full Text] [PDF]
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T. Kamitani, H. P. Nguyen, and E. T. H. Yeh Preferential Modification of Nuclear Proteins by a Novel Ubiquitin-like Molecule J. Biol. Chem., May 30, 1997; 272(22): 14001 - 14004. [Abstract] [Full Text] [PDF]
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D. Halverson, M. Baum, J. Stryker, J. Carbon, and L. Clarke A Centromere DNA-binding Protein from Fission Yeast Affects Chromosome Segregation and Has Homology to Human CENP-B J. Cell Biol., February 10, 1997; 136(3): 487 - 500. [Abstract] [Full Text] [PDF]
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A. F. Pluta and W. C. Earnshaw Specific Interaction between Human Kinetochore Protein CENP-C and a Nucleolar Transcriptional Regulator J. Biol. Chem., August 2, 1996; 271(31): 18767 - 18774. [Abstract] [Full Text] [PDF]
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Y. Mao, S. D. Desai, and L. F. Liu SUMO-1 Conjugation to Human DNA Topoisomerase II Isozymes J. Biol. Chem., August 18, 2000; 275(34): 26066 - 26073. [Abstract] [Full Text] [PDF]
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X. Long and L. C. Griffith Identification and Characterization of a SUMO-1 Conjugation System That Modifies Neuronal Calcium/Calmodulin-dependent Protein Kinase II in Drosophila melanogaster J. Biol. Chem., December 22, 2000; 275(52): 40765 - 40776. [Abstract] [Full Text] [PDF]
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K.-S. Dai and C.-C. Liew A Novel Human Striated Muscle RING Zinc Finger Protein, SMRZ, Interacts with SMT3b via Its RING Domain J. Biol. Chem., June 22, 2001; 276(26): 23992 - 23999. [Abstract] [Full Text] [PDF]
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Y. Takahashi, T. Kahyo, A. Toh-e, H. Yasuda, and Y. Kikuchi Yeast Ull1/Siz1 Is a Novel SUMO1/Smt3 Ligase for Septin Components and Functions as an Adaptor between Conjugating Enzyme and Substrates J. Biol. Chem., December 21, 2001; 276(52): 48973 - 48977. [Abstract] [Full Text] [PDF]
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I. M. Cheeseman, C. Brew, M. Wolyniak, A. Desai, S. Anderson, N. Muster, J. R. Yates, T. C. Huffaker, D. G. Drubin, and G. Barnes Implication of a novel multiprotein Dam1p complex in outer kinetochore function J. Cell Biol., December 24, 2001; 155(7): 1137 - 1146. [Abstract] [Full Text] [PDF]
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I. M. Cheeseman, D. G. Drubin, and G. Barnes Simple centromere, complex kinetochore: linking spindle microtubules and centromeric DNA in budding yeast J. Cell Biol., April 15, 2002; 157(2): 199 - 203. [Abstract] [Full Text] [PDF]
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